Professional Research Assistant

Job description


University of Colorado School of Medicine

University of Colorado | CU Anschutz Medical Campus

School of Medicine, Department of Pediatrics

Section of Cardiology

Professional Research Assistant (PRA)

Position #655844 – Requisition #19173

* Applications are accepted electronically ONLY at *

The University of Colorado Denver l Anschutz Medical Campus seeks individuals with demonstrated commitment to creating an inclusive learning and working environment.  We value the ability to engage effectively with students, faculty and staff of diverse backgrounds.

The Department of Pediatrics, Section of Cardiology has an opening for a full-time (unclassified) Professional Research Assistant position.

The University of Colorado Anschutz Medical Campus is a public education, clinical and research facility serving 4,500 students, and a world-class medical destination at the forefront of life-changing science, medicine, and healthcare. CU Anschutz offers more than 42 highly rated degree programs through 6 schools and colleges, and receives over $500 million in research awards each year. We are the single largest health professions education provider in Colorado, awarding nearly 1,450 degrees annually. Powered by our award-winning faculty, renowned researchers and a reputation for academic excellence, the CU Anschutz Medical Campus drives innovation from the classroom to the laboratory to the delivery of unparalleled patient care. Read CU Anschutz Quick Facts here.

Nature of Work:

A full-time Professional Research Assistant (PRA) position is available in the area of pediatric heart failure research. The PRA will have a key role in conducting a variety of experiments exploring the molecular changes present in children with cardiomyopathy and congenital heart disease. Laboratory techniques include, but are not limited to, RNA and protein extraction, cDNA synthesis, RT-qPCR, Western blotting, ELISA assays, maintaining primary cell culture, and peripheral blood mononuclear cell isolation. Various models of heart failure have been developed, including primary cardiomyocyte cell culture as well as rodent models. Experiments are also performed utilizing human samples (Institutional Review Board approved Pediatric Cardiac Tissue and Blood Bank).

Examples of Work Performed:

  • Human induced pluripotent stem cell cardiomyocyte (hiPSC-CM) differentiation and maturation
  • Transfection and CRISPR/Cas9 gene editing of hiPSC-CM
  • RNA, DNA, and protein extraction from cardiac tissue and cardiomyocyte cell culture
  • Peripheral blood mononuclear cell isolation and serum processing from human whole blood samples
  • Cardiac protein quantification through Western blotting
  • RT-qPCR in a 384-well format
  • ELISA assays
  • Reagent preparation
  • Organization and cataloging of human samples (in RedCap), antibodies, and chemicals
  • Laboratory supply ordering and invoice tracking
  • PLEASE NOTE: Candidates may be responsible for travel expenses related to the interview process and any relocation expenses, if applicable.

    Salary and Benefits:


    Salary is commensurate with skills and experience. The University of Colorado offers a full benefits package. Information on University benefits programs, including eligibility, is located at Employee Services.


    University of Colorado Denver | Anschutz Medical Campus is dedicated to ensuring a safe and secure environment for our faculty, staff, students and visitors. To assist in achieving that goal, we conduct background checks for all new employees prior to their employment.


    The Immigration Reform and Control Act requires that verification of employment eligibility be documented for all new employees by the end of the third day of work. Alternative formats of this ad are available upon request for persons with disabilities.


    Your total compensation goes beyond the number on your paycheck. The University of Colorado provides generous leave, health plans and retirement contributions that add to your bottom line.



    Total Compensation Calculator:


    Diversity and Equity:


    Please click here for information on disability accommodations:


    Office of Equity:


    The University of Colorado Denver | Anschutz Medical Campus is committed to recruiting and supporting a diverse student body, faculty and administrative staff. The university strives to promote a culture of inclusiveness, respect, communication and understanding. We encourage applications from women, ethnic minorities, persons with disabilities and all veterans. The University of Colorado is committed to diversity and equality in education and employment.

    QualificationsMinimum Qualifications:

    • A baccalaureate degree in an appropriate scientific field (biology, molecular biology, biochemistry, physiology, pharmacology, genetics or similar)
    • Two years of full-time molecular biology laboratory bench research experience
    Applicants must meet minimum qualifications at the time of hire.

    Conditions of Employment:

    • Must successfully pass a drug test through Children’s Hospital Colorado
    • Must be willing and able to pass a national criminal background check
    Preferred Qualifications:

    • Hands-on experience handling pediatric cardiac tissue and processing pediatric blood samples
    • Training in molecular techniques with a good theoretical understanding and experience in interpretation of results
    • Cell culture techniques, including maintaining primary cell cultures
    • Laboratory management skills 

    • Ability to work in the laboratory on weekends, as dictated by experiment schedule
    • Excellent organizational and documentation skills
    • Detail-oriented and responsive
    • Ability to collaborate and work effectively in a team environment
    • Self-motivated and scientifically inquisitive
    • Problem-solver
    • Ability to communicate effectively, both in writing and orally

    Job Category: Faculty
    Primary Location: Aurora
    Schedule: Full-time
    Posting Date: Sep 14, 2020
    Unposting Date: Ongoing




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    Job No:
    Posted: 9/18/2020
    Application Due: 10/17/2020
    Work Type: