Biological Scientist III - Pediatrics Cell/Molecular Therapy
Job no: 514355
Work type: Staff Full-Time
Location: Main Campus (Gainesville, FL)
Categories: Agriculture/Life Science/Vet Medicine, Research/Scientific/Grants
Department:29092300 - MD-PEDS-CELL / MOLEC THERP DIV
Biological Scientist III - Pediatrics Cell/Molecular TherapyJob Description:
The Senior Biological Scientist position described herein performs research in the laboratory for the Department of Pediatrics, Division of Cellular and Molecular Therapy. Works under general supervision. Relies on significant experience and judgment to plan and accomplish goals. Defines goals for a given research project in conjunction with faculty and/or other staff. A wide degree of creativity and latitude is expected. Very familiar with a variety of the field’s concepts, practices, and procedures. Performs a variety of complicated tasks.
Responsibilities to include:
Histology Analysis: Collecting organs and other tissue and embedding the specimens for sectioning either with a microtome or cryotome (for frozen sections). Following preparation of slides with sectioned tissue, staining with standard histology stains such as H&E, as well as with immunohistochemistry will be done. Participating in the image capture and analysis of tissue following intervention studies. This includes treating mice, collecting tissue and processing it for analysis.
Molecular Techniques and Analysis: Molecular cloning, Plasmid purification, Primer design, RNA and DNA isolation, isolating RNA and performing real-time PCR, and PCR genotyping of transgenic mouse lines. Participating in in vitro studies exploring novel vectors in various cells. This may include expansion/maintenance of cell lines and analysis of transfection/infection. Performing protein analysis by isolating protein of interest from cell or tissue lysates and performing/analyzing western blots.
Animal Studies: Handling mice for setting up timed matings, weaning animals and maintaining colonies. Participating in treating animals with pharmacological agents or AAV vectors either through i.p., i.m., or i.v. injections. Other duties with the mice include biodistribution and gene expression studies.Expected Salary:
$50,000 - $55,000 annually; commensurate within this range based on qualifications and experience.Minimum Requirements:Bachelor's degree in biology or a closely related field and seven years of relevant experience; or a Master's degree in biology or a closely related field and five years of relevant experience; Doctoral degree in biology or a closely related field and two years of relevant experience.Preferred Qualifications
- Experience in gene therapy research (Specifically AAV).
- Animal colony management and handling of rodents experience.
- Advanced degree in related scientific field.
- Working knowledge of basic laboratory techniques.
- Must be extremely knowledgeable in laboratory procedures and must be meticulous in recording, tracking & reporting of data collected.
To be considered for this position, please include a cover letter, resume, and a three professional references with your online application form.
Work schedule will be Monday through Friday, 8:00am to 5:00pm. Nights & weekends as needed for on-going research.
This is a time-limited position.
This requisition has been reposted. Previous applicants are still under consideration and need not apply.
Application must be submitted by 11:55 p.m. (ET) of the posting end date.Health Assessment Required:Yes
Advertised: 14 Sep 2020 Eastern Daylight Time
Applications close: 28 Sep 2020 Eastern Daylight Time