Professional Research Assistant

Job description


University of Colorado School of Medicine

University of Colorado | CU Anschutz Medical Campus

School of Medicine, Department of Pediatrics

Section of Genetics & Metabolism

Professional Research Assistant (PRA)

Position #00734773– Requisition #19055


* Applications are accepted electronically ONLY at *


The University of Colorado Denver l Anschutz Medical Campus seeks individuals with demonstrated commitment to creating an inclusive learning and working environment.  We value the ability to engage effectively with students, faculty and staff of diverse backgrounds.


The Department of Pediatrics, Section of Genetics & Metabolism has an opening for a full-time (unclassified) Professional Research Assistant (PRA) position.


The University of Colorado Anschutz Medical Campus is a public education, clinical and research facility serving 4,500 students, and a world-class medical destination at the forefront of life-changing science, medicine, and healthcare. CU Anschutz offers more than 42 highly rated degree programs through 6 schools and colleges, and receives over $500 million in research awards each year. We are the single largest health professions education provider in Colorado, awarding nearly 1,450 degrees annually. Powered by our award-winning faculty, renowned researchers and a reputation for academic excellence, the CU Anschutz Medical Campus drives innovation from the classroom to the laboratory to the delivery of unparalleled patient care. Read CU Anschutz Quick Facts here.


Nature of Work:

Research studies in mitochondrial functional assays. The person will grow fibroblasts and evaluate them with testing of mitochondrial biology such as enzyme activities of respiratory chain complexes, ATP synthesis, western blotting, and high resolution respirometry, and microscopy of in situ mitochondrial functional assays. These tests will be applied to normal and to mitochondrial disease specific samples. The person may also be involved in studies proving the pathogenicity of new genetic causes of mitochondrial disease, using tests as outlined above. Verification of a genetic defect via sequencing may be needed, and transfection with a normal and mutant gene copy to prove pathogenicity may also be pursued. The person will maintain the collection of cell lines from patients with metabolic disorders, in particular mitochondrial disorders at the laboratory.


Examples of Work Performed:

  • Tissue culture of fibroblasts and other eukaryotic cells. Testing of cells for mycoplasma contamination. Developing primary cultures of fibroblasts or lymphoblasts
  • Perform enzyme assays of mitochondrial enzymes
  • Perform western blotting of various kinds (denaturing and non-denaturing)
  • Measure ATP synthesis using HPLC
  • Perform high resolution respirometry
  • Perform labeling of mitochondria with reagents such as mitotracker, mitochondrial membrane potential probes, or COX histochemistry and perform confocal microscopy and analysis
  • Create genetic vector and perform transfection experiments
  • PLEASE NOTE: Candidates may be responsible for travel expenses related to the interview process and any relocation expenses, if applicable.


    Salary and Benefits: Salary iscommensurate with skills and experience. The University of Colorado offers afull benefits package. Information on University benefits programs, includingeligibility, is located at Employee Services.

     University ofColorado Denver | Anschutz Medical Campus is dedicated to ensuring a safe andsecure environment for our faculty, staff, students and visitors. To assist inachieving that goal, we conduct background checks for all new employees priorto their employment.

     The ImmigrationReform and Control Act requires that verification of employment eligibility bedocumented for all new employees by the end of the third day of work. Alternativeformats of this ad are available upon request for persons with disabilities.

     Yourtotal compensation goes beyond the number on your paycheck. The University ofColorado provides generous leave, health plans and retirement contributionsthat add to your bottom line.

      Benefits: Calculator:

     Diversityand Equity:

     Pleaseclick here for information on disability accommodations:

     Office of Equity: TheUniversity of Colorado Denver | Anschutz Medical Campus is committed torecruiting and supporting a diverse student body, faculty and administrativestaff. The university strives to promote a culture of inclusiveness, respect,communication and understanding. We encourage applications from women, ethnicminorities, persons with disabilities and all veterans. The University ofColorado is committed to diversity and equality in education and employment.

    QualificationsMinimum Qualifications:

    • Bachelor of Science or higher in biology, genetics, biochemistry or a related field.
    • Experience working in a laboratory
    Applicants must meetminimum qualifications at the time of hire.

    Conditions of Employment:

    • Must be willing and able to successfully pass a 10-panel drug test
    • Must be willing and able to successfully pass a national criminal background check
    Preferred Qualifications

    • Experience with functional testing in mitochondrial biology such as high resolution respirometry or Western blotting.  
    • Experience with mitochondrial assays, such as enzyme assays, HPLC
    • Experience with microscopic exams
    • Experience with molecular analysis such as sequencing, vector generation, transfection of cells

    • Tissue culture for eukaryotic cells in particular human fibroblasts
    • The ability to work independently within the lab on established mitochondrial functional assays
    • The ability to write lab methods and scientific papers.
    • Organizational skills to maintain a log of cell lines with results
    • Excellent communications skills using the English language
    • Able to work in a research group context including flexibility in scheduling hours given current access restrictions 

    Job Category: Faculty
    Primary Location: Aurora
    Schedule: Full-time
    Posting Date: Aug 26, 2020
    Unposting Date: Ongoing




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    Job No:
    Posted: 8/27/2020
    Application Due: 9/18/2020
    Work Type: