#102377 Staff Research Associate
UCSD Layoff from Career Appointment: Apply by 10/21/19 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.
Special Selection Applicants: Apply by 10/31/19. Eligible Special Selection clients should contact their Disability Counselor for assistance.DESCRIPTION
Under supervision, the Staff Research Associate will independently work with transgenic mouse models, mice perfusion, dissection, and tissues collection. Responsible for organization and maintenance of large mouse colony comprised of multiple transgenic mouse lines. Will independently conduct mouse experiments including euthanasia, sample collection and processing, data collection and analysis.
Performs routine molecular biology techniques such as bacteria transformation, plasmid purification, RNAs extraction, PCR, qPCR, Western Blot, and restriction digest. Under instruction use aseptic technique on Mammalian cell cultures independently; conduct experiments including planning experiments and analyzing results. Laboratory management includes monitoring, ordering and receiving supplies, prepare biohazards and radioactive waste for collection according to safety regulations, and maintaining a functional, clean and orderly lab.MINIMUM QUALIFICATIONS
Theoretical knowledge of a biological science such as Biology, Microbiology or a related field. Knowledge of the basic science of Cardiovascular Diseases, including familiarity with general medical problems and terminology.
Demonstrated experience in general techniques for mouse colony maintenance.
Experience in the management and modifications of the breeding colony including genotyping, euthanasia, breeding colony establishment, weaning, and maintaining inventory.
Proven experience in general techniques in molecular biology. Experience in standard and repetitive molecular biology techniques, including DNA/RNA preparation.
Proven experience in general laboratory techniques. Experience and ability to use various lab instruments including balance and autoclave.
Ability to prepare solutions at proper concentrations, operate analytical balances and perform accurate calculations.
Demonstrated ability to measure, weigh, and mix chemicals. Ability to order and maintain lab equipment and supplies.
Knowledge of library usage to locate journals in stacks and from other sources, and ability to copy specific articles for research.
Excellent organizational and time management skills. Ability to work with minimum supervision and to work closely and collaboratively with others including ability to follow written and oral instructions.
Knowledge of laboratory safety precautions including appropriate clothing and shoes, safe handling of chemicals, use of protective items such as gloves and goggles. Familiarity with Material Safety Data Sheets (MSDSs).
Employment is subject to a criminal background check.
Must be willing to work with mice.