#101958 Asthma and Allergy Staff Research Associate

La Jolla, CA
Oct 02, 2019
Institution Type
Four-Year Institution

UCSD Layoff from Career Appointment: Apply by 10/02/19 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.

Special Selection Applicants: Apply by 10/14/19. Eligible Special Selection clients should contact their Disability Counselor for assistance.


The Department of Medicine is responsible for fulfilling the teaching, research, and clinical missions of the University of California, San Diego and employs 500+ full-time salaried faculty members and 150+ academic appointees. Staff personnel include 800+ staff in 9 bargaining units, students, and volunteers.

There are over 250 clinical faculties practicing at multiple sites and hospitals. Professional fee billings for the department exceed $50 million with collections of over $19 million per year. The total annual budget is approximately $200 million, which includes contract and grant revenues, clinical practice and medical center funds, and other state and private endowment funds.

The Division of Hospital Medicine in the Department of Medicine at the University of California, San Diego is comprised of a dynamic group of 36 faculty hospitalists and nocturnist physicians providing inpatient care for the UC San Diego Health medical centers in Hillcrest and La Jolla. Hospitalists are engaged in teaching and are leaders in quality improvement throughout the medical center.

This laboratory studies mechanisms of asthma and allergy with a view to novel therapeutic development. Experiments are performed independently and as part of a team.

The Asthma and Allergy Staff Research Associate will function as a senior staff research associate responsible for independently performing laboratory bench experiments to identify novel targets and pathways in asthma. The Staff Research Associate will perform in vitro experiments with human lymphocytes (T cells and innate lymphoid cells) that include simulations, differentiations, antibody blockade assays as well as co-culture with human airway epithelial cells and smooth muscle cells.

The candidate must have extensive experience with flow cytometry, qPCR, ELISA, and lymphocyte in vitro studies (preferably T cells) which are necessary for these studies. Further, some studies will require processing of human FACS sorted lymphocyte subpopulations for RNASeq. Evaluates ongoing experimental procedures on a continuous basis for efficacy and data significance. Procedures and data will be evaluated for use in manuscripts and grants. Performs advanced statistical analysis and organize and manage weekly lab meetings along with PI.

Finally, the Staff Research Associate will independently make innovative contributions pertaining to experimental design and methodology by reviewing published scientific data in journals, as well as online using search engines and subject-specific websites to make recommended changes and standards to current protocols and practices.

  • Theoretical knowledge of a life science as is typically required by a Bachelor's Degree or graduate Degree in a field related of research such as biology, molecular biology, immunology, and biochemistry; or an equivalent combination of education and experience..

  • Proven extensive laboratory experience in addition to coursework to include ELISA, flow cytometry, RT-PCR, and processing human cells from peripheral blood.

  • Direct laboratory experience working with human, preferably lymphocyte, in vitro studies and performing differentiation and stimulation assays.

  • Demonstrated experience with magnetic separation of cells and processing cells for FACS sorting and RNASeq.

  • Demonstrated experience with immunofluorescent staining on cultured cells.

  • Demonstrated experience performing standard laboratory techniques (pipetting, weighing).

  • Strong experience in record keeping, paying close attention to detail. Good organizational skills to order and maintain lab equipment and supplies.

  • Excellent computer skills and ability to learn new computer programs. Proven knowledge of Excel, Word, Outlook. Demonstrated data entry experience.

  • Proven experience with anonymous database design to record human sample data in accordance with IRB guidelines.

  • Able to perform data calculations and perform advanced statistical analysis from experimental results and use appropriate computer software to include programs such as Excel, Prism, online ELISA software and flow cytometry software FlowJo.

  • Demonstrated experience providing input to experimental design.

  • Demonstrated experience assisting with manuscript preparation.

  • Strong familiarity with human subjects protocols, able to edit protocols, amendments, and craft correspondence between IRB and laboratory.

  • Able to make innovative contributions pertaining to experimental design and methodology by reviewing published scientific data in journals, and using search engines and subject-specific websites to make recommended changes and standards to current protocols and practices.

  • Excellent written and verbal communication skills needed to interact professionally and effectively in the work environment, and effectively document research procedures. Ability to read, comprehend and discuss research materials.

  • Effective interpersonal skills. Ability to work with a variety of personnel from different laboratories.

  • Knowledge of and ability to follow established Safety and Biohazardous Material Guidelines.

  • Advanced degree such as an MS or PhD is highly preferred.
  • Employment is subject to a criminal background check.

  • Must be willing to work with human peripheral blood cells and airway structural cells.

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