#101726 Staff Research Associate I
UCSD Layoff from Career Appointment: Apply by 09/17/2019 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.
Special Selection Applicants: Apply by 09/26/2019. Eligible Special Selection clients should contact their Disability Counselor for assistance.DESCRIPTION
Incumbent will assist Principal Investigator in research to understand the role of plant hormones auxin and gibberelin in plant growth and development using genetic and molecular approaches. Under supervision, the incumbent will perform experiments in plant genetics and molecular biology, including DNA sub-cloning, DNA extraction from bacteria and Arabidopsis, PCR, DNA and protein electrophoresis, and confocal laser scanning microscopy. Incumbent will also maintain and cross Arabidopsis lines. Additional duties to include: oversee the maintenance of lab inventory, including ordering of supplies and sending out stocks, and train and provide work direction to undergraduate student workers. When appropriate, the incumbent will assist researchers in preparing scientific manuscripts for publication, and participate in lab meetings and journal clubs.
Must be willing and able to work safely with radioactive and hazardous materials.
Ability to see observable physical or biochemical characteristics.
Must be willing to work overtime during weekdays and weekend as required by experiments.
Theoretical knowledge of genetics and molecular biology acquired through Bachelor degree in biology or related field, and/or combination of education and relevant lab experience.
Demonstrated experience with Arabidopsis thaliana, including genetic crosses and screens; demonstrated experience in phenotypic analyses of mutant lines.
Proven experience in recombinant DNA techniques, Southern blots and Western blots, Sub-clone constructs using classical cloning, site-directed mutagenisis, and Gateway recombination cloning.
Demonstrated experience in transmission electron microscopy, field emission scanning electron microscopy, laser scanning confocal microscopy, and chromatin immunoprecipitation assays.
Proven experience with basic molecular biology laboratory equipment such as centrifuges, microscopes, balances, pH meter, micro-pipetting equipment, etc.
Demonstrated ability to record scientific data in a well-organized laboratory notebook. Demonstrated organizational skills to keep clear and accurate records.
Knowledge of specialized scientific terminology related to research; knowledge of library databases such as PubMed to locate journal articles electronically and in print.
Demonstrated ability to work independently or collaboratively on a research project. Ability to maintain a high level of motivation to contribute to cutting edge research, and seek and learn new experimental methods as required.
Demonstrated skills to read and follow established lab protocols and work effectively with minimal instruction.
Experience and knowledge of safety procedures involved in working with radioisotopes and radioactive material.
Knowledge of Material Safety Data Sheets, and hazardous communication and Injury and Illness Prevention Program. Knowledge of laboratory safety precautions, including use of protective items such as goggles, coats, etc.
Demonstrated ability to train and provide work direction to undergraduate student workers in various lab techniques and procedures (safety precautions, preparation of media, dishwashing, equipment usage, laboratory maintenance, etc.).
Experience and skill with MS Office programs, Adobe Photoshop, spreadsheet, and other computer software, including DNA and protein analysis; willingness and ability to update and increase PC skills and knowledge as needed.
Basic knowledge of accounting, billing practices, purchasing, and reimbursement procedures. Ability to proactively and appropriately facilitate the maintenance of all lab supplies and equipment, maintain steady levels of lab supplies, and organize purchasing records.
Good eyesight (normal or corrected) to distinguish subtle variations in analysis of phenotypes.
Demonstrated ability in using tact, diplomacy, discretion, and flexibility with diverse personalities and situation. Demonstrated ability to work as part of a research team, and interact effectively with collaborating labs and others in scientific community.
- Job offer is contingent upon satisfactory clearance based on Background Check results.