#101578 Research Associate
This is a UC San Diego internal recruitment open to UCSD and UCSD Health System staff only.
UCSD Layoff from Career Appointment: Apply by 09/17/2019 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.
Special Selection Applicants: Apply by 09/19/2019. Eligible Special Selection clients should contact their Disability Counselor for assistance.DESCRIPTION
The Moores Cancer Center is one of just 49 in the United States to hold a National Cancer Institute (NCI) designation as a Comprehensive Cancer Center. As such, it ranks among the top centers in the nation conducting basic and clinical cancer research, providing advanced patient care and serving the community through outreach and education programs.
The Cancer Center's mission is to translate promising scientific discoveries into new and better options for the care of patients with cancer. The Center supports one of the broadest ranges of cancer activities in the nation – from fundamental and translational cancer research in molecular genetics and advanced molecular therapeutics, and from population studies of cancer incidence to community education about cancer prevention and risk reduction.
Under supervision, perform tasks and assays to support research on tumor inflammation. Responsibilities include assisting with routine molecular biological, biochemical, and cell biological techniques as well as in vivo animal experiments that assess the role of myeloid cells in cancer. Assist with endothelial, myeloid and tumor cell culture, DNA and RNA isolation from culture cells and from tissues, immunoblotting and quantitative RT-PCR for genes and proteins that regulate myeloid cell adhesion, migration and gene and protein expression in vitro and in vivo.
Assist with assays that evaluate T cell mediated killing of tumor cells in vitro and in vivo. Assist in performing bone marrow transplant. Assist with isolating bone marrow and blood cells for analysis using flow cytometry, immunoblotting, gene expression and cell adhesion assays. Assist in protein biochemical techniques such as protein purification, protein labeling, and protein analysis. Perform basic cryosectioning of tissues, immunohistochemistry, fluorescent microscopy, ELISA assays, and SDS PAGE analyses. Maintain and prepare accurate records of all experiments.MINIMUM QUALIFICATIONS
Theoretical knowledge in molecular biology, biochemistry, physiology and cell biology.
Experience assisting in performing bone marrow transplant. Isolate bone marrow and blood cells for analysis by flow cytometry, immunoblotting, gene expression and cell adhesion assays.
Proven experience with in vivo experiments including assisting in the injection of growth factors and tissue harvesting of mice and in performing minor surgery.
Basic experience with protein analysis techniques including protein determination and protein separation techniques.
Demonstrated ability to perform basic molecular biological techniques, including DNA purification and cloning.
Ability to work with endothelial, myeloid and tumor cell culture, DNA and RNA isolation from culture cells and from tissues, immunoblotting and quantitative RT-PCR for genes and proteins that regulate myeloid cell adhesion, migration and gene and protein expression in vitro and in vivo.
Ability to use basic laboratory equipment, including centrifuges, spectrophotometers, balances pH meters and electrophoresis equipment.
Ability to learn basic tissue culture techniques, including sterile technique, cell adhesion and migration assays.
Willingness and ability to carry out mouse and chicken embryo studies.
Ability to learn to carry out radioactive work.
Experience with personal computers, including word processing and spreadsheets.
Strong communication skills (verbal and written) needed to interact professionally and effectively in work environment. Effectively and accurately document research procedures. Ability to read, comprehend, and discuss research materials.
- Employment is subject to a criminal background check.
- Must be willing to frequently handle small animals.
- Able to work occasional nights and weekends.