#101509 Laboratory Research Associate

Location
La Jolla, CA
Posted
Sep 11, 2019
Institution Type
Four-Year Institution

This position will remain open until a successful candidate has been identified.

UCSD Layoff from Career Appointment: Apply by 9/12/2019 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.

Special Selection Applicants: Apply by 9/23/2019. Eligible Special Selection clients should contact their Disability Counselor for assistance.

DESCRIPTION

The Moores Cancer Center is one of just 49 in the United States to hold a National Cancer Institute (NCI) designation as a Comprehensive Cancer Center. As such, it ranks among the top centers in the nation conducting basic and clinical cancer research, providing advanced patient care and serving the community through outreach and education programs. The Cancer Center's mission is to translate promising scientific discoveries into new and better options for the care of patients with cancer. The Center supports one of the broadest ranges of cancer activities in the nation – from fundamental and translational cancer research in molecular genetics and advanced molecular therapeutics, and from population studies of cancer incidence to community education about cancer prevention and risk reduction.

The Laboratory Research Associate will be expected to contribute to the independent design, conduct, and analysis of experiments and their results and also preparation, presentation, and publication of results under the direction of the Principal Investigator (PI). Make significant innovative contributions pertaining to the design and overall direction of scientific research projects and propose possible changes or new directions in the course of the investigations. Read and evaluate scientific articles relevant to current research studies, finding new sources of information. The incumbent will be responsible for applying or adapting scientific techniques and assisting with the planning and scheduling of research procedures, recording the results of studies, compiling and analyzing data including statistical analyses, and preparing charts and graphs. Scientific intellect especially as it pertains to the immunology and molecular biology is essential. The incumbent will independently maintain and manage an animal breeding colony consisting of approximately 150 cages of animals, maintain laboratory equipment, order supplies as needed and assist laboratory management to provide support for students, postdocs and general laboratory operations.

Multiple myeloma, a tumor of plasma cells, ranks as the second most common type of blood cancer. Our laboratory is very active in the development of novel myeloma animal models to test novel targeted therapies and immunotherapies. We are also studying human primary cells obtained from myeloma patients, and our passion is to understand antigen-presenting cells (dendritic cells) and other myeloid regulatory cells operating at the myeloma tumor site.

MINIMUM QUALIFICATIONS
  • Advanced theoretical knowledge and/or Bachelor's Degree in immunology, molecular biology, and/or related work experience.

  • Demonstrated strong experience handling and working with small animals, including demonstrated experience conducting mouse surgeries, experiments, and injections. Knowledge in conducting euthanization of small animals.

  • Strong demonstrated experience performing DNA cloning and manipulation, lentiviral preps and transduction.

  • Strong experience with protein biochemistry techniques; including quantification, ELISA assays, SDS PAGE, Western and Immunoblotting, phosphorylations and gel electrophoresis.

  • Demonstrated strong experience performing multi-parametric flow cytometry and cell sorting.

  • Demonstrated strong experience in cellular immunology techniques, such as cytotoxicity assays, ELISPOT, cytokine detection.

  • Demonstrated strong experience and knowledge in coordinating major mouse experiments, such as planning injection and drug treatment dosing schedule.

  • Strong demonstrated experience using laboratory equipment such as centrifuges, spectrophotometers, balances, pH meters, autoclaves and electrophoresis equipment.

  • Demonstrated experience performing sterile eukaryotic cell culture techniques, PCR and agarose gel electrophoresis.

  • Demonstrated experience using histological and immunohistochemical techniques to evaluate protein localization in fixed cells or tissues.

  • Demonstrated experience performing light, fluorescence and confocal microscopy techniques to visualize protein expression in tissue sections.

  • Demonstrated experience in proper handling of chemical and biohazard materials. Demonstrated experience with sterile techniques.

  • Experience with computer software such as Excel and Prism to analyze data, store results and compute raw data. Demonstrated experience of computer applications such as Word, Excel and PowerPoint. Internet search and software analysis capabilities.

  • Demonstrated experience ordering and maintaining lab equipment and supplies.

  • Experience in troubleshooting problems for quality control on every immunochemical and histochemical procedures.

  • Strong experience and background in mouse/human and cellular immunology.

PREFERRED QUALIFICATIONS
  • CRISPR/Cas9 experience.

  • CyTOF mass cytometry experience.

SPECIAL CONDITIONS
  • Employment is subject to a criminal background check.

  • Must be willing and able to work with hazardous materials (biological and chemical).

  • Must be willing and able to work with small animals.

  • Must be able to work various hours and locations based on business needs.

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