Senior Research Associate
Posting DetailsPosting Details Posting NumberS03924PWorking TitleSenior Research AssociateDepartmentResearch Molecular Medicine AdminAbout the University of Georgia
The University of Georgia (UGA), a land-grant and sea-grant university with statewide commitments and responsibilities is the state’s oldest, most comprehensive, and most diversified institution of higher education (http://www.uga.edu/). UGA is currently ranked among the top 20 public universities in U.S. News & World Report. The University’s main campus is located in Athens, approximately 65 miles northeast of Atlanta, with extended campuses in Atlanta, Griffin, Gwinnett, and Tifton. UGA was founded in 1785 by the Georgia General Assembly as the first state-chartered University in the country. UGA employs approximately 1,800 full-time instructional faculty and more than 7,600 full-time staff. The University’s enrollment exceeds 36,000 students including over 27,500 undergraduates and over 8,500 graduate and professional students. Academic programs reside in 17 schools and colleges, as well as a medical partnership with Augusta University housed on the UGA Health Sciences Campus in Athens.About the College/Unit/DepartmentCollege/Unit/Department websitePosting TypeExternalRetirement PlanTRS or ORPEmployment TypeEmployeeBenefits EligibilityBenefits EligibleFull/Part timeFull TimeWork ScheduleAdditional Schedule Information
Monday – Friday; some nights and weekends may be requiredAdvertised SalarynegPosting Date09/06/2019Open until filledYesClosing DateProposed Starting Date11/01/2019Special Instructions to ApplicantsLocation of VacancyAthens Area EEO Policy Statement
The University of Georgia is an Equal Opportunity/Affirmative Action employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, ethnicity, age, genetic information, disability, gender identity, sexual orientation or protected veteran status.Position Information Classification TitleResearch Professional ADPay GradeFLSAExemptJob FamilyFTE1.0Minimum Qualifications
Requires at least a baccalaureate degree in the field. Please contact your Human Resources office for an evaluation of education/experience in lieu of the required minimum qualifications.Preferred Qualifications
The candidate will be doing original research, including designing experiments, developing protocols, co-supervising (together with Dr. Ivanova) junior postdocs and graduate students, presenting at the meetings and writing research papers. The candidate will also be involved in experiments.
This Senior Research Associate will work on the NIH grant R01GM130847 entitled “Deciphering genetic and epigenetic regulatory logic of germ layer differentiation with manifold learning” (PI-Krishnaswamy, Yale University) on which Dr. Ivanova serves as Co-Investigator with a subcontract to UGA. This is a collaborative project which combines computational and experimental work which would be carried out by the Krishnaswamy lab at Yale and the Ivanova lab at UGA. The UGA subcontract budget does not fund personnel salaries but covers all reagents and supplies needed for this work.
The goal of this project is to understand the gene regulatory logic that guides differentiation of human ES
cells along specific cell lineages. The following analyses will be performed:
1) We will generate an extended 60-day EB differentiation timecourse that captures both early and late stage germ layer derivatives. This data will be used by the Krishnaswamy lab to construct gene regulatory networks guiding lineage differentiation.
2) We will develop antibody cocktails for purification of key differentiation stages along specific branches. We will use these markers to FACS purify the end-point and intermediate differentiation stages for epigenetic mapping. Cell identity of the sorted populations will be confirmed by bulk RNA-seq and correlation with scRNA-seq data. 3) We will acquire ChIP-seq and ATAC-seq data from endpoint and intermediate differentiation stages. ATAC-seq will be performed to define accessible genomic regions. H3K4me3, H3K27ac, H3K4me1 and H3K27me3 marks will be profiled by ChIP-seq to identify active and repressed promoter and enhancer regions in each sorted population.
4) We will validate computational models of germ layer differentiation generated by the Krishnaswamy lab via a timed shRNA screen measured with scRNA-seq to observe loss of lineages with knockdown of transcription factors, chromatin modifiers and other critical combinations of genes found in the analysis.
Normal for laboratory workIs driving a responsibility of this position?Does this position have operation, access, or control of financial resources?NoDoes this position require a P-Card? NoIs having a P-Card an essential function of this position? NoDoes this position have direct interaction or care of children under the age of 18 or direct patient care?NoDoes this position have Security Access (e.g., public safety, IT security, personnel records, patient records, or access to chemicals and medications)NoCredit and P-Card policy
Be advised a credit check will be required for all positions with financial responsibilities. For additional information about the credit check criteria, visit the UGA Credit Background Check website.Duties/Responsibilities Duties/Responsibilities
Develop antibody cocktails for purification of key differentiation stages along specific germ layer branches. FACS purify the end-point and intermediate differentiation stages for epigenetic mapping. Acquire ChIP-seq and ATAC-seq data from these cells.Percentage of time40 Duties/Responsibilities Duties/Responsibilities
Collect single cell RNA-seq data from wild type and genetically modified human ESCs differentiating as embryoid bodies. The genetic modifications will include knock-out and overexpression of genes predicted to have the key roles on differentiation of specific germ layers.Percentage of time50 Duties/Responsibilities Duties/Responsibilities
Prepare oral or written reports, write manuscripts, and maintain records of the research carried out as part of this projectPercentage of time10Posting Specific Questions
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