Staff Research Associate I (9613C) - 27480

San Francisco
Aug 09, 2019
Institution Type
Four-Year Institution

Staff Research Associate I (9613C) - 27480
About Berkeley
The University of California, Berkeley, is one of the world's most iconic teaching and research institutions. Since 1868, Berkeley has fueled a perpetual renaissance, generating unparalleled intellectual, economic and social value in California, the United States and the world. Berkeley's culture of openness, freedom and acceptance—academic and artistic, political and cultural—make it a very special place for students, faculty and staff.

Berkeley is committed to hiring and developing staff who want to work in a high performing culture that supports the outstanding work of our faculty and students. In deciding whether to apply for a staff position at Berkeley, candidates are strongly encouraged to consider the alignment of the Berkeley Workplace Culture with their potential for success at

Application Review Date
The First Review Date for this job is: August 20, 2019

Departmental Overview
The Nayak lab studies the physiology and evolution of methanogenic archaea. The SRA will assist with performing routine molecular biology experiments towards the lab's goals of generating randomly barcoded transposon mutant libraries. Primary duties of the research technician will include performing standard molecular biology techniques such as PCR for DNA amplification, gel electrophoresis and Gibson assembly reactions. Other duties will include routine microbiology cultivation techniques and maintenance of lab equipment, reporting results and assisting in drafting manuscripts. Ideal candidates would have prior experience in working with microorganisms and performing transposon mutagenesis.

Molecular biology - Routine experiments including but not limited to:
  • Designing primers to perform PCR amplification of DNA
  • Gel electrophoresis
  • Plasmid DNA extraction and DNA purification
  • DNA ligation using the Gibson assembly technology
  • Sample preparation for sequencing
  • Analysis of sequencing data.

    Cultivation of Microorganisms - Routine experiments including but not limited to:
  • Preparation of growth media and other reagents
  • Using the spectrophotometer to measure optical density and assay growth rates
  • Setting up liposome mediated transformation assays with Methanosarcina
  • Maintaining the anaerobic growth chambers

    Required Qualifications
  • B.S. in microbial biology and at least one year of experience working at the laboratory bench and/or equivalent experience/training.
  • The candidate should be familiar with routine lab instruments such as a thermocycler, measuring balance, spectrophotometer, and a microcentrifuge.
  • Familiarity with molecular biology techniques such as PCR, DNA extraction, plasmid purification and gel electrophoresis.
  • Familiarity with sterile techniques for the cultivation of microorganisms and follow procedures to prepare growth media, reagents and buffers.

    Preferred Qualifications
  • Prior experience cultivating microorganisms and performing transposon mutagenesis
    is strongly preferred.

    Salary & Benefits
    This position is a non-exempt, bi-weekly paid position. Hourly pay for this role is commensurate with experience within the range of $21.36 - $23.27, Steps 1.0 - 5.0.

    For information on the comprehensive benefits package offered by the University visit:

    How to Apply
    Please submit your cover letter and resume as a single attachment when applying.

    Other Information
    This position is governed by the terms and conditions in the agreement for the Research Support Professionals Unit (RX) between the University of California and the University Professional and Technical Employees (UPTE). The current bargaining agreement manual can be found at:

    Conviction History Background
    This is a designated position requiring fingerprinting and a background check due to the nature of the job responsibilities. Berkeley does hire people with conviction histories and reviews information received in the context of the job responsibilities. The University reserves the right to make employment contingent upon successful completion of the background check.

    Equal Employment Opportunity
    The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or protected veteran status. For more information about your rights as an applicant see:
    For the complete University of California nondiscrimination and affirmative action policy see:

    To apply, visit

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