This is a 100% career position for eight (8) months which may be extended based on the needs of the department.

The UC San Diego Department of Bioengineering consists of ~25 ladder rank faculty, ~600 enrolled undergraduate students, ~280 graduate students, ~45 postdoctoral scholars, and ~115 staff positions. The department is consistently ranked as one of the top departments of Bioengineering in the world. It has a $10M annual allocation of State and University funds and holds a multi-million research portfolio (17.7 MIL in total contract & grant expenditures), including awards (but not limited) from the NIH, NSF, other federal flow through, and gifts/private funding.

The Department of Bioengineering is one of six departments in the nationally ranked Jacobs School of Engineering. It enjoys close collaborations with the UC San Diego School of Medicine, the Institute of Engineering in Medicine (IEM), the Scripps Research Institute, the Sanford Burnham Institute, and the Salk Institute for Biological Studies, among other Divisions and Programs on campus.

The UC San Diego Department of Bioengineering offers the following graduate degrees: Masters of Engineering, Masters of Science, combined Bachelors of Science/Masters of Science, Ph.D. and (in conjunction with the UC San Diego School of Medicine) a combined M.D./Ph.D. degree program.

Under supervision, the Research Assistant will perform live-cell imaging analysis, genetic engineering, synthetic biology and their application in developing molecular biosensors capable of visualizing epigenetic dynamics of single live cells. The incumbent will utilize the Fluocell image analysis software to quantify live cell image data.

The incumbent will also perform standard laboratory procedures with Human Pancreatic Cells and other experimental procedures. Perform cell culture laboratory procedures using cell biology techniques, including FRET live-cell imaging of pancreatic cancer cells. Perform a number of standard repetitive laboratory procedures with human pancreatic cancer cells and t-cells.

Use techniques of molecular biology and microscopy to conduct a limited number of experimental procedures and achieve results. Effectively use microscopes available to visualize phase contrast and fluorescence images. Adapt standard experimental protocols in tissue culture to meet the needs of the research projects. Perform general lab duties including maintaining lab website and computer repair. Engage in training to learn new techniques.

• Proven experience with live-cell image analysis: segmentation, tracking, and quantification.

• Knowledge of computer programming, data structure, and algorithm design. Ability to write and analyze computer programs in MATLAB and Java.

• Experience with various computer software, hardware, standard office equipment and lab equipment. Ability to maintain and repair simple problems related with lab computers and printers.

• Experience with sterile techniques and mammalian cell culture practices. Knowledge of lab computers related to uses, maintenance, and repair.

• Demonstrated knowledge of sterile cell culture techniques.

• Demonstrated knowledge of molecular biology, DNA techniques and protein biochemistry.

• Demonstrated ability to use microscopy to perform experiments.

• Theoretical knowledge of cellular biology, bioengineering, or related field acquired through a Bachelor's Degree; or an equivalent combination of education and experience.

• Ability to perform repetitive laboratory experimental procedures. Ability to follow experimental protocols, and learn new procedures.

• Ability to use mathematical skills to analyze and develop statistical data. Acute attention to detail. Ability to be resourceful and capable of handling the changing needs of the research lab.

• Strong control of standard English in written and spoken form. Ability to communicate effectively in writing, electronically, and by spoken word with a variety of people from vendors to faculty.

• Good interpersonal communication skills that facilitate professional, effective, and courteous interaction with diverse population including employees, faculty, researchers, and outside agencies. Ability to effectively present information and recommendations.

• Excellent multi-tasking and organization skills to conduct a variety of tasks in an extremely fast-paced environment. Ability to work independently and as part of a large, diverse group.