Laboratory Specialist in Pharmacology (Sharlow)

Job description

Job location: Charlottesville, VA


Employment Type: Full-time
Posted data: 2019-04-18
Req: R0005272
The Department of Pharmacology, at the University of Virginia School of Medicine, is seeking a Laboratory Specialist to assist investigators with the development of high content screening (HCS) assays for Alzheimer’s Disease. The project focuses on the development of a model system for amyloid β-mediated cell cycle reentry using human iPS neuronal progenitor and neurons. The Lab Specialist working under the supervision of Dr. Elizabeth Sharlow, Principal investigator, and will be expected to provide research support related to HCS assay development, validation and implementation. RT-PCR, fluorescence microscopy, mammalian cell culture, Western blotting, and/or sterile technique and/or additional molecular biology experience will be necessary. The incumbent will meet regularly with the Principal Investigator to discuss experimental setup, results and data analysis. The incumbent will also present data in lab meetings.

To learn more about Dr. Sharlow and the Fiske Drug Discovery Laboratory, please visit:
https://pharm.virginia.edu/faculty/primary-faculty/?facbio=1&id=26001
https://pharm.virginia.edu/fiske/

A Bachelor’s degree in biology, chemistry, biochemistry, molecular biology or a related discipline and 3 years of laboratory experience in an academic or industrial laboratory setting is required. Familiarity with basic laboratory equipment (i.e., centrifuges, pH meters, pipettors) and the ability to perform calculations is expected. Experience with Microsoft Office (i.e., Word, Powerpoint and Excel) is also expected. Experience with GraphPad Prism, statistical analysis, and exposure to high content imaging methodologies is preferred. The ideal candidate will be a highly motivated individual with excellent organizational and communication skills.

This position is restricted and contingent upon the continuation of funding.

This position will remain open until filled.

TO APPLY:
EXTERNAL APPLICANTS: please apply through Workday at the following URL, https://uva.wd1.myworkdayjobs.com/UVAJobs , and search for requisition # R0005272. Complete an application online and attach a cover letter, CV/resume, and contact information for three references (name, email address, telephone number, address).
INTERNAL UVA APPLICANTS: please apply through your Workday Home page, search “Find Jobs”, and search for requisition # R0005272. Complete an application online and attach a cover letter, CV/resume, and contact information for three references (name, email address, telephone number, address).

Lab Specialist employees provide technical lab support by performing complex lab and research related tasks. They act as specialists on research methods and equipment functions specific to investigations. They have the authority to adapt methods and procedures to meet the needs of research. They are distinguished from Lab Technicians by the types and duration of assignments, as well as the skills and expertise necessary to serve as resources on non-routine problems. Lab Specialist employees perform daily activities without supervision; their longer-term assignments require greater oversight. Lab Specialist employees often train others on work processes and policies.   Responsibilities will include:

1. Experimental Work  (Approximately 80%)

  • Perform and help optimize experimental  assays related to assay development and HCS image capture. 
  • Use sterile technique for mammalian tissue culture of human iPS neuronal progenitor cells and neurons.
  •  Isolate protein lysates and perform western blotting.
  • Perform immunofluorescence.
  • Capture images on the Perkin Elmer Operetta.
  •  Perform data analysis and visualization
  • Present data at laboratory meetings
  • Perform reagent, test plate and sample transfer to University of Virginia core facilities for experimentation/evaluation.

2. Laboratory Maintenance & General Duties  (Approximately 15%)

  • Maintain a clean laboratory area and assist with overall laboratory maintenance
  • Make buffers and other reagents
  • Maintain a laboratory notebook
  • Be available to assist principal investigator as needed

3. Ordering, Purchasing, & Shipping  (Approximately 5%)

  • Process purchases for the lab
  • Return damaged or incorrect items
  • Receive packages

4. Other duties as assigned.

MINIMUM REQUIREMENTS:

Education:    A Bachelor’s degree in biology, chemistry, biochemistry, molecular biology or a related discipline is required.

Experience:  The Candidate will require the following to be successful in this position:

  • Three (3) years of laboratory experience in an academic or industrial laboratory setting is required, which may include experience gained while a student.
  • Provide research support related to HCS assay development, validation and implementation.  
  • RT-PCR, fluorescence microscopy, mammalian cell culture, Western blotting, and/or sterile technique and/or additional molecular biology experience will be necessary. 
  • Familiarity with basic laboratory equipment (i.e., centrifuges, pH meters, pipettors) and the ability to perform calculations is expected.
  • Experience with Microsoft Office (i.e., Word, Powerpoint and Excel) is also expected.
  • Experience with GraphPad Prism, statistical analysis, and exposure to high content imaging methodologies is preferred.
  • The ideal candidate will be a highly motivated individual with excellent organizational and communication skills.

PHYSICAL DEMANDS:

 

This position can spend extensive periods of time standing and squatting while conducting experiments or caring for animals. Also requires keyboarding and other bench work and animal procedures requiring finer finger manipulation.  

The University of Virginia, including the UVA Health System and the University Physician’s Group are fundamentally committed to the diversity of our faculty and staff.  We believe diversity is excellence expressing itself through every person's perspectives and lived experiences.  We are equal opportunity and affirmative action employers. All qualified applicants will receive consideration for employment without regard to age, color, disability, gender identity, marital status, national or ethnic origin, political affiliation, race, religion, sex (including pregnancy), sexual orientation, veteran status, and family medical or genetic information.

 

 

 

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Job No:
Posted: 4/19/2019
Application Due: 6/11/2019
Work Type:
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