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RESEARCH TECHNICIAN II - Human Vaccine Institute

Employer
Duke University
Location
Durham, NC

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Administrative Jobs
Academic Affairs, Research Staff & Technicians
Employment Type
Full Time
Institution Type
Four-Year Institution

Job Details


diversity employer



RESEARCH TECHNICIAN II - Human Vaccine Institute
Duke Human Vaccine Institute - Admin

RESPONSIBILITIES:
  • Execute cultures of primary cells with impeccable sterile techniques and adherence to safety protocols.
  • Proficiently manipulate clinical samples (whole blood, biopsies, PBMCs, etc.), primary cells and established cell lines, culture supernatants and stocks of infectious agents.
  • Prepare culture media, reagents and solutions according to specifications.
  • Isolate primary cells using fluorescent-activated or magnetic-activated cell sorting from specimens collected from individuals, non-human primates and other small animals.
  • Perform functional high-throughput screenings (HTS) of biological samples (e.g. serum), cell culture supernatants and purified antibodies (e.g. ELISA, neutralization assays, etc.).
  • Coordinate the acquisition of reagents (both internally and externally) needed to complete assigned experiments.
  • Operate and maintain laboratory equipment, including complex automation systems.
  • Maintain laboratory workspace organized, clean and compliant with safety regulations.
  • Perform DNA and RNA extraction, RT-PCR, nested PCR and overlapping PCR.
  • Produce and purify monoclonal antibodies from transiently transfected cell cultures.
  • Document, compile, analyze, and draw preliminary conclusions from experimental results.
  • Maintain detailed documentation of executed work, ensure data integrity and accessibility, and generate timely, concise and precise reports.
  • Confer with PI and laboratory personnel to review work assignments and develop experimental plans
  • Adhere to BSL-2 and BSL-3 working conditions and safety regulations.
  • Perform other duties as requested by the PI.

Work Schedule
You must be willing and available to work flexible hours which may include later start and end times with the understanding that work hours may need to be modified to ensure a project/task has reached a logical and acceptable stopping point to maintain the integrity of the science.

Preferred Skills and Experience
  • Bachelor's degree in a related field.
  • Experience in handling cell/tissue cultures with strong record of performing sterile techniques
  • Experience in performing biochemical and immunological measurements of biological variables (e.g. ELISA, bead-based multiplex assays, flow cytometry).
  • Experience in performing RNA extraction, RT and PCR.
  • Experience in protein purification and identification techniques.
  • Willing to work with human specimens
  • Must be detail-oriented, focused, well organized and approach procedures with critical thinking and strong experimental record keeping.
  • Must be able to manage multiple and rapidly changing priorities with firm deadlines in a highly dynamic and competitive environment, execute experiments and generate deliverables in a timely fashion.
  • Must be able to work effectively and follow procedures independently and in a team environment as required.
  • Must have excellent communication skills.
  • Previous experience in managing high throughput screening (HTS) and automated systems.
  • Previous experience in working in fast paced environments managing multiple priorities simultaneously.
  • Previous experience working with biological and chemical materials.


Requisition Number
401545062

Location
Durham

Duke Entity
MEDICAL CENTER

Job Code
951 RESEARCH TECHNICIAN II

Job Family Level
08

Full Time / Part Time
FULL TIME

Regular / Temporary
Regular

Shift
First/Day

Minimum Qualifications
Duke University is an Affirmative Action/Equal Opportunity Employercommitted to providing employment opportunity without regard to anindividual's age, color, disability, gender, gender expression, genderidentity, genetic information, national origin, race, religion, sex,sexual orientation, or veteran status.
Duke aspires to create a community built on collaboration, innovation,creativity, and belonging. Our collective success depends on the robustexchange of ideas—an exchange that is best when the rich diversity ofour perspectives, backgrounds, and experiences flourishes. To achievethis exchange, it is essential that all members of the community feelsecure and welcome, that the contributions of all individuals arerespected, and that all voices are heard. All members of our communityhave a responsibility to uphold these values.
Essential Physical Job Functions:Certain jobs at Duke University and Duke University Health System mayinclude essential job functions that require specific physical and/ormental abilities. Additional information and provision for requests forreasonable accommodation will be provided by each hiring department.

Education

Work requires a bachelor's degree in botany, biology, zoology or otherscience related scientific field.

Experience

None required above education/training requirement.OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE

Auto req ID

108846BR

Duke University is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, genetic information, gender, gender expression, gender identity, national origin, race, religion, sexual orientation, or veteran status.

Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essential job functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.

PI106972382

Organization

Read our Diversity Profile History

Duke University was created in 1924 by James Buchanan Duke as a memorial to his father, Washington Duke. The Dukes, a Durham family that built a worldwide financial empire in the manufacture of tobacco products and developed electricity production in the Carolinas, long had been interested in Trinity College. Trinity traced its roots to 1838 in nearby Randolph County when local Methodist and Quaker communities opened Union Institute. The school, then named Trinity College, moved to Durham in 1892, where Benjamin Newton Duke served as a primary benefactor and link with the Duke family until his death in 1929. In December 1924, the provisions of indenture by Benjamin’s brother, James B. Duke, created the family philanthropic foundation, The Duke Endowment, which provided for the expansion of Trinity College into Duke University.Duke Campus

As a result of the Duke gift, Trinity underwent both physical and academic expansion. The original Durham campus became known as East Campus when it was rebuilt in stately Georgian architecture. West Campus, Gothic in style and dominated by the soaring 210-foot tower of Duke Chapel, opened in 1930. East Campus served as home of the Woman's College of Duke University until 1972, when the men's and women's undergraduate colleges merged. Both men and women undergraduates now enroll in either the Trinity College of Arts & Sciences or the Pratt School of Engineering. In 1995, East Campus became the home for all first-year students.

Duke maintains a historic affiliation with the United Methodist Church.

Home of the Blue Devils, Duke University has about 13,000 undergraduate and graduate students and a world-class faculty helping to expand the frontiers of knowledge. The university has a strong commitment to applying knowledge in service to society, both near its North Carolina campus and around the world.

Mission Statement

Duke Science"James B. Duke's founding Indenture of Duke University directed the members of the University to 'provide real leadership in the educational world' by choosing individuals of 'outstanding character, ability, and vision' to serve as its officers, trustees and faculty; by carefully selecting students of 'character, determination and application;' and by pursuing those areas of teaching and scholarship that would 'most help to develop our resources, increase our wisdom, and promote human happiness.'

“To these ends, the mission of Duke University is to provide a superior liberal education to undergraduate students, attending not only to their intellectual growth but also to their development as adults committed to high ethical standards and full participation as leaders in their communities; to prepare future members of the learned professions for lives of skilled and ethical service by providing excellent graduate and professional education; to advance the frontiers of knowledge and contribute boldly to the international community of scholarship; to promote an intellectual environment built on a commitment to free and open inquiry; to help those who suffer, cure disease, and promote health, through sophisticated medical research and thoughtful patient care; to provide wide ranging educational opportunities, on and beyond our campuses, for traditional students, active professionals and life-long learners using the power of information technologies; and to promote a deep appreciation for the range of human difference and potential, a sense of the obligations and rewards of citizenship, and a commitment to learning, freedom and truth.Duke Meeting

 “By pursuing these objectives with vision and integrity, Duke University seeks to engage the mind, elevate the spirit, and stimulate the best effort of all who are associated with the University; to contribute in diverse ways to the local community, the state, the nation and the world; and to attain and maintain a place of real leadership in all that we do.”

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