RESEARCH TECHNICIAN II
RESEARCH TECHNICIAN II
Biostat & Bioinformatics Chairs Office
Perform a variety of complex technical duties involved in conducting high-throughput genomics experiments to support research efforts in the Reddy Lab at Duke University. The work performed will be part of a large-scale collaborative project involving the Ciofani, Gersbach and Crawford labs also at Duke. The successful applicant will be under the direction and supervision of the postdoc leading the project and will report primarily to him. He/she will be given ample opportunities to further develop his/her independent research skills. Details of the Reddy Lab can be viewed at http://reddylab.org
Major tasks to be performed will include: the management of a mouse colony through breeding and genotyping; mouse handling and dissections to isolate primary cells; large scale primary culture using sterile technique; expansion and differentiation of cells in preparation of experiments; transfection of cells using both transient and viral approaches; characterization and purification of cells using magnetic and fluorescence assisted cell sorting; targeted genome and epigenome editing using CRISPR/Cas9 based tools; isolation of nucleic acids through chromatin immunoprecipitation, RNA and DNA purifications; generation of high-throughput sequencing libraries for genome wide analysis. Additional tasks relating to molecular biology will include growth and preparation of electrocompetent bacteria; cloning, transformation, and isolation of plasmid DNA from E. coli.
Perform routine laboratory tasks related to the above goals including preparation of reagent stocks, cell growth media, and buffers; ordering and stocking sufficient supplies and equipment for performing experiments; cleaning and maintain laboratory equipment.
Review results and quality control from completed experiments; record results in lab notebooks; communicate results in weekly update meetings with project leads. Option to contribute in weekly journal clubs and group meetings.
Perform other tasks and special projects as assigned.
951 RESEARCH TECHNICIAN II
Job Family Level
Full Time / Part Time
Regular / Temporary
Duke University is an Affirmative Action/Equal Opportunity Employercommitted to providing employment opportunity without regard to anindividual's age, color, disability, gender, gender expression, genderidentity, genetic information, national origin, race, religion, sex,sexual orientation, or veteran status.
Duke aspires to create a community built on collaboration, innovation,creativity, and belonging. Our collective success depends on the robustexchange of ideas—an exchange that is best when the rich diversity ofour perspectives, backgrounds, and experiences flourishes. To achievethis exchange, it is essential that all members of the community feelsecure and welcome, that the contributions of all individuals arerespected, and that all voices are heard. All members of our communityhave a responsibility to uphold these values.
Essential Physical Job Functions:Certain jobs at Duke University and Duke University Health System mayinclude essential job functions that require specific physical and/ormental abilities. Additional information and provision for requests forreasonable accommodation will be provided by each hiring department.
Work requires a bachelor's degree in botany, biology, zoology or otherscience related scientific field.
None required above education/training requirement.OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE
Auto req ID
Duke University is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, genetic information, gender, gender expression, gender identity, national origin, race, religion, sexual orientation, or veteran status.
Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essential job functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.