Laboratory Specialist Senior

Job description

Working Title: Laboratory Specialist Senior

Position Type: University Managerial and Professional Staff

Department: Department of Cell Biology

Location: Charlottesville

Posting Date: 11-08-2018

Posting Summary:
The Department of Cell Biology, at the University of Virginia, School of Medicine, is currently seeking a Lab Specialist Senior. The successful candidate will play an integral role in setting-up the new Laboratory of Dr. Karen Hirschi. This position would start on or about January 1, 2019, under the supervision of Dr. Douglas DeSimone, and, on or about April 1, 2019, when the lab is in full operation, will fall under the supervision of Dr. Hirschi.

The Hirschi Lab will study vascular and hematopoietic development using the mouse embryo model system, and translate mechanisms of morphogenesis to the production of human vascular and blood cells from human pluripotent stem cells for tissue engineering and regenerative medicine projects. The Lab Specialist Senior, initially, will have duties and responsibilities involving lab set-up. Once in operation, the Lab Specialist Senior will be expected to perform the following techniques needed for his/her research project: flow cytometry; confocal microscopy; mammalian cell culture; cell-based assays including blood cell and stem cell colony forming assays; DNA and RNA transfection; virus and pseudovirus production and purification; small-scale protein production and purification; SDS-PAGE and western blotting; molecular cloning including primer design, PCR mutagenesis, DNA gel electrophoresis, restriction enzyme analysis, DNA production and DNA purification; and ELISA. In addition, the incumbent will be responsible for learning, applying and teaching (other lab members) bioinformatics approaches to analyze data sets derived from RNA and DNS sequencing studies. The incumbent will also be responsible for general lab organization and maintenance, mouse colony maintenance, ordering all supplies for the lab, arranging shipments of reagents and mouse lines, arranging lab meetings, preparation and characterization of biological reagents, and (semi)-independent experiments as directed by the PI or senior lab personnel.

This position requires a requires a Terminal Degree or a Master's Degree in Biology, Biochemistry, Microbiology, or a related field, with at least 8 years of post-Master's Degree lab experience.

This position is restricted and is contingent upon the continuation of funding.

This position will remain open to applications until filled.

To apply for this position, please visit and search on posting # 0624464 or visit: Complete an Application online, attach a cover letter, resume/CV, and contact information for three references.

For information related to this posting, please contact Dr. Douglas DeSimone, at [email protected] For information related to the application process please contact Lois Rowsey at [email protected]

The University of Virginia is an equal opportunity and affirmative action employer. Women, minorities, veterans and persons with disabilities are encouraged to apply.

Required Knowledge, Skills and Abilities:
1. Ability to independently perform laboratory experiments and independently document the results.
2. Excellent oral and written communication and organizational skills.
3. Demonstrated knowledge of specific areas of research subject matter expertise.
4. Ability to synthesize information, provide advice, and address needs independently.
5. Knowledge of general lab procedures, basic safety precautions, and lab equipment.
6. Proven ability to work independently and as part of a team, Work with PI to accomplish overall lab goals. Ability to lead, train and supervise.
7. Familiarity with grants and budgeting procedures as well as advanced knowledge of relevant university, state and federal policies and regulations.

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Job No:
Posted: 11/9/2018
Application Due: 1/4/2019
Work Type: