Associate Professional Specialist

Location
Princeton, NJ
Posted
Oct 13, 2018
Institution Type
Four-Year Institution

Princeton University

We are looking for a highly-motivated mouse embryo microinjectionist/lab manager for a new laboratory at Princeton University studying preimplantation mouse development. This position provides the opportunity to: *Create and run a small state-of-the-art transgenic laboratory and learn cutting edge CRISPR/Cas9-based genome editing methods. *Work in an innovative, interdisciplinary and scientifically stimulating surrounding. *Take part in further improving CRISPR/Cas9-based genome editing methods, develop new targeting strategies and explore working with non-conventional model species. Responsibilities: *Help set up new embryo micromanipulation lab for generation of genetically modified embryos and mouse models using CRISPR/Cas9 editing - primarily serving the research needs of the Posfai lab, and also providing transgenic services for several other mouse user labs in the department *Prepare CRISPR/Cas9 reagents *Carry out embryo collection, microinjection, electroporation, embryo transfer surgeries and post-operative care *Efficient mouse colony management, including breeding, superovulation injections, and plug checks *Independently plan and schedule transgenic experiments *Diligent record keeping and use of animal database *Order and maintain supplies for the embryo micromanipulation lab Qualifications: *Doctorate in Molecular Biology, Genetics, Biochemistry, Veterinary Medicine, Biotechnology or related area. *Minimum 2 years' experience with transgenic techniques such as pronuclear microinjections and embryo transfer surgeries *Good hands and flexibility to learn new microinjection techniques *Experience breeding genetically modified mouse lines *Proficiency in molecular biology techniques (plasmid preparations, PCR, basic cloning, in vitro transcription *Demonstrated ability to assume and take full ownership of responsibilities *Excellent communication skills This is a two-year term position with the possibility of renewal. This position is subject to the University's background-check policy. Applicants must apply by November 11, 2018 at 11:59 p.m. EST at https://www.princeton.edu/acad-positions/position/9362.

PI104872046

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