RESEARCH TECHNICIAN II - BEHAVIORAL NEUROLOGY
RESEARCH TECHNICIAN II - BEHAVIORAL NEUROLOGY
Performs a variety of complex technical duties involved in conducting laboratory tests, experiments and determinations in support of Duke University research operations.
RESPONSIBILITIES:• Performs a variety of complex technical duties involved in conducting physical,chemical, biological and other research laboratory tests, experiments and determinations to obtain data for research purposes;compile, analyze, document and draw tentative conclusions from experimental results.• Confer with the principal investigator to review work assignments and develop plans for research experiments; make minor modifications to existing laboratory procedures and techniques to meet the needs of the particular equipment as required.• Review laboratory methods manual, scientific journals, abstracts, and other literature for information applicable to research experiments.• Determine and setup instruments, material, and apparatus and operate laboratory equipment required for specific tests.• Obtain, prepare, mount and stain tissue, blood and other substances for microscopic examination.• Prepare culture media, chemicals, reagents and solutions.• Maintain sufficient inventory of material, supplies, and equipment for performance of duties; clean and maintain laboratory equipment.• Review work of lower-level laboratory personnel within designated areas and assist in training new personnel.• Perform other related duties incidental to the work described herein.• Review completeness or accuracy of the work of others• Give recommendations, advice or guidance to others• Review expenses related to materials and supply, and confirm the grant-budget monthly reports.• Generating reports of results• Create and maintain laboratory records. (protocols and biological materials)
SKILLS: Knowledge/experience in various methods of molecular biology, analysis of gene expression,genetics,genomics, cell biology, and bioinformatics. Samples receipt and handling, iPSC technology, genome editing, siRNA, real-time PCR, RNA handling, RT- PCR, qPCR, LCM, FACS, cell culture, transfections, luciferase reporter assays, GFP reporter assay and immunofluorescence, RNA and DNA extraction, cloning, plasmids construction, plasmids preps, protein extraction and analysis, nuclear extraction, chromatin immuno-precipitation, electrophoretic mobility shift assay, DNA-binding protein purification, sequence analysis, genotyping, characterizing animal models, wide genomic comparison analysis across evolution, computer analysis of TF binding sites, bioinformatic tools for genomic analysis.
951 RESEARCH TECHNICIAN II
Job Family Level
Full Time / Part Time
Regular / Temporary
Duke University is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex,sexual orientation, or veteran status.
Duke aspires to create a community built on collaboration, innovation,creativity, and belonging. Our collective success depends on the robust exchange of ideas—an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.
Essential Physical Job Functions:Certain jobs at Duke University and Duke University Health System may include essential job functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.
Work requires a bachelor's degree in botany, biology, zoology or other science related scientific field.
None required above education/training requirement.OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE
Auto req ID
Duke University is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, genetic information, gender, gender expression, gender identity, national origin, race, religion, sexual orientation, or veteran status.
Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essential job functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.