Job description

diversity employer

Cell Biology

Occupational Summary

Conduct a variety of highly technical experimental procedures and other tasks, including imaging with the latest confocal microscopes, involved in the planning, development and implementation of a research project at Duke University. Work Performed Perform experimental procedures involving multi-step or complex laboratory methods to collect data and new information for a Duke University research project, by operating specialized, highly technical laboratory instruments and equipment, where necessary. Devise or modify experimental techniques for tests and analyses. Maintain detailed laboratory notes, documenting procedures, results and interpretations of findings, in compliance with the Duke University laboratory SOP and Duke University requirements. Evaluate and interpret data, and prepare reports and analyses of progress, adverse trends, recommendations and conclusions. Prepare specimens for live and fixed cell imaging, and collect images using light and fluorescence microscopes available in the Duke University laboratory and the Duke University LMCF, including recent/latest model confocal and other microscopes. Stain fixed tissue with antibodies and localize antibodies by imaging. Analyze live and fixed images using image analysis software, including ImageJ/FIJI, to obtain data for publication. As needed, obtain TEM images in the Duke University Electron Microscopy Facility, analyze images and prepare images for publication. Use molecular biology techniques to perform experiments needed to carry out the research project, including DNA/RNA extraction, plasmid preps, PCR, gel electrophoresis, molecular cloning, transformation, Southern blots, protein preps and Western blots. Perform cell culture, transfection, live imaging or fixation of cultured cells, and antibody staining of cells, as necessary. Contribute to the development of procedures involving CRISPR/Cas9 mutagenesis and gene modification. Assist with Drosophila culture as needed, including stock maintenance and genetic crosses. Review journals, abstracts and scientific literature to keep abreast of new developments and to obtain information regarding previous experiments to aid in the design and development of new procedures and techniques. Confer with principal investigator in determining specific experiments to be included in the research protocol and to discuss the interpretation of results. Supervise, train and instruct students or other personnel in laboratory techniques and procedures. Perform laboratory management duties, including ordering of supplies and maintaining inventories, laboratory safety and hazardous substance requirements, and recombinant DNA compliance.
Perform other related duties incidental to the work described herein.

Requisition Number


Duke Entity

Job Code

Job Family Level


Full Time / Part Time

Regular / Temporary


Minimum Qualifications
Duke University is an Affirmative Action/Equal Opportunity Employercommitted to providing employment opportunity without regard to anindividual's age, color, disability, gender, gender expression, genderidentity, genetic information, national origin, race, religion, sex,sexual orientation, or veteran status.
Duke aspires to create a community built on collaboration, innovation,creativity, and belonging. Our collective success depends on the robustexchange of ideas—an exchange that is best when the rich diversity ofour perspectives, backgrounds, and experiences flourishes. To achievethis exchange, it is essential that all members of the community feelsecure and welcome, that the contributions of all individuals arerespected, and that all voices are heard. All members of our communityhave a responsibility to uphold these values.
Essential Physical Job Functions:Certain jobs at Duke University and Duke University Health System mayinclude essential job functions that require specific physical and/ormental abilities. Additional information and provision for requests forreasonable accommodation will be provided by each hiring department.


Work requires a bachelor's degree in botany, biology, zoology or otherdirectly related scientific field.


Work requires two years of research experience. A related master'sdegree may offset required years of experience on a 1:1 basis, e.g., atwo year master's degree in lieu of two years of experience.OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE

Auto req ID


Duke University is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, genetic information, gender, gender expression, gender identity, national origin, race, religion, sexual orientation, or veteran status.

Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essential job functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.





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Job No:
Posted: 6/11/2018
Application Due: 8/10/2018
Work Type: