RESEARCH SPECIALIST C
Duties: The Simpkins laboratory in the Ovarian Cancer Research Center has the goal to identify novel therapies in the lab and bring them to the clinic for the treatment of ovarian cancer. The Research Specialist should have extensive experience with cell culture and mice. Candidate will be required to manage mouse experiments-handle mice, care for mice, perform surgical procedures which include transplantation of human tumor to mice ovaries (training will be provided) to develop patient-derived xenograft (PDX), measure tumors using ultrasound (training will be provided), resect tumors and bio-banking. Candidate will isolate DNA, RNA and protein from PDX tumors. Must be able to maintain very organized records of experiments, maintain cell line and human tissue and PDX data base. The individual will be in charge of keeping animal protocols up to date.
The Research Specialist should have experience in cellular/ molecular biology. Candidate should have experience in preparing protein lysates, running western blots. Experience running flow cytometry is preferred. Proficiency in molecular biology techniques such a transfection/transduction, PCR are preferred. Ability to trouble shoot is required. Responsibilities will include growing primary tumor cultures, establishing cell lines, performing in vitro protein assays. We will be testing cells with a multi-compound drug-screen. The candidate should be able to work independently.
Qualifications: The minimum of a Bachelor's degree and 3 to 5 years of experience or an equivalent combination of education and experience. (Advanced degree preferred.)
Must have experience working with mice. Should be able to perform major surgical procedures and survival surgeries along with and tumor resection. Must be able to maintain organized laboratory data. Must be able to work well with others and is a team player. Should be willing to help train others. Occasional work on the weekends and evenings may be necessary. Flexibility with hours is required.
Experience in tissue culture such as growing human primary cells, ability to transfect/transduce cells, ability to perform western blots, and flow cytometry. Should be able to perform DNA/RNA analysis.
Preferred but not required qualifications: experience growing primary tumor cultures, ability to perform cell line/tumor implants within the ovarian bursa, immunology background and experience in humanized mouse models preferred.
Position is contingent upon funding
Reference Number: 40-28554
Salary Grade: 026
Employment Type: Exempt
Org: WM-Ctr for Res on Reprod and Women's Health
Job Family: I-Technical/Professional Research