Research Assistant - Piedrahita Lab
Position Number: 00106904
Primary Function of Organization Unit: The Department of Molecular Biomedical Sciences consists of 45 faculty who have a diversity of research and teaching interests, including clinical faculty, affording students the opportunity to participate in research projects with a direct clinical correlation to veterinary and/or human medicine. New, state-of-the-art research facilities are available including a transgenic mouse facility, permitting the opportunity for graduate students to participate in research projects related to mouse and animal genomics. A number of graduate programs are available to students interested in working with faculty in the Department of Molecular Biomedical Sciences. The Comparative Biomedical Sciences graduate program in CVM consists of several different "tracks", allowing students to focus their research in the areas of cell biology, infectious diseases, pharmacology, pathology, and population medicine. Other graduate programs with which departmental faculty are affiliated include programs in immunology, physiology, and functional genomics.
Essential Job Duties: The position involves project management of multiple concurrent projects, analysis of data and assistance in determining the next steps of such projects relating to somatic cell nuclear transfer (cloning) and gene editing of swine. Project management also includes independently supporting, developing, designing, and executing fundamental research activities for a single or multiple research project(s) and suggesting options for improving quality and recommending solutions. This position will supervise a team responsible for generating transgenic swine. As part of those responsibilities the candidate will participate in the development of pigs by somatic cell nuclear transfer (cloning). The incumbent is responsible for all aspects related to this component of the laboratory including planning, scheduling and record keeping of ongoing projects. Oocyte collection, in vitro maturation, somatic cell nuclear transfer, activation of oocytes, embryo transfer, and tissue collection on various points of gestation, surgical techniques, isolation of fetal fibroblast cell lines, transfection and selection of stable cell lines. In addition ability to work with pigs in a gnobiotic environment is needed. Works closely with staff at lake wheeler swine unit to manage transgenic herd, by managing and monitoring pregnancy, gestation, farrowing, weaning, finishing, and breeding of pigs developed by the lab. In addition, the incumbent assists with grant and manuscript writing and contributes to research publications, reports and manuscripts. The position requires interaction with outside collaborators.
Minimum Education/Experience: Must have Bachelor's degree in biological science, preferably biochemistry, genetics, molecular biology, or related field plus 3 or more years of independent research experience, or substantative relevant independent experience and education. Experience with general molecular biology skills (including genomic DNA/RNA isolation and QC, PCR) required.
Department Required Skills: Prior research experience. Strong verbal and written communication skills. Excellent organization skills with strong attention to detail. Ability to meet tight deadlines, goal oriented and high level of motivation Ability to work individually and as part of a team. Competency with Microsoft office and data analysis software Excellent science writing skills. Must be able to significantly contribute to grant and manuscript writing. General knowledge of somatic cell nuclear transfer procedure, ability quickly learn to perform all technical aspects of the cloning process including cloning media preparation, oocyte collection and maturation, polar body search, enucleation, cell injection, activation, in-vitro culture, and transfer of embryos. Ability to manage pigs under gnobiotic conditions including all aspects of cage preparation and pig management. Must be able to lift up to 30 pounds.
Preferred Experience, Skills, Training/Education: Knowledge and skills to adjust and calibrate instrumentation and equipment used in somatic cell nuclear transfer as needed with limited supervision. Knowledge and experience in cell culture procedures involved in isolation of fetal fibroblast cell lines, and screening and analysis of those lines. Experience running a gnobiotic pig facility. Extensive experience working with pigs.
Necessary Licenses and Certifications: N/A
AA/EOE: NC State University is an equal opportunity and affirmative action employer. All qualified applicants will receive consideration for employment without regard to race, color, national origin, religion, sex, gender identity, age, sexual orientation, genetic information, status as an individual with a disability, or status as a protected veteran.
Individuals with disabilities requiring disability-related accommodations in the application and interview process, please call 919-515-3148. Final candidates are subject to criminal & sex offender background checks. Some vacancies also require credit or motor vehicle checks. If highest degree is from an institution outside of the U.S., final candidates are required to have their degree verified at "www.wes.org":http//wes.org. Degree must be obtained prior to start date.
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