RESEARCH SPECIALIST B
Duties: The Research Specialist is to prepare macromolecular components needed for our ongoing experiments, which includes basic sub-cloning and mutagenesis of protein expression constructs. Growth and induction of protein expression constructs in both bacterial and insect cells. Growth and harvesting of plasmids used for high yield defined DNA sequences. Large scale polymerase chain reaction for generating defined DNA sequences. Affinity and conventional chromatography steps for protein and DNA purification. Assessment of protein purity and abundance by spectroscopic and gel-based techniques. Perform biochemical and biophysical experiments. Biochemical and biophysical assays: Hydrogen/deuterium exchange experiments to assess the backbone dynamics of purified proteins in isolation or assembled with their macromolecular binding partners. Aid in general lab functions.
In addition to the above, the Research Specialist C is expected to rapidly learn and then independently execute and interpret the following experiments:
Assessment of macromolecular complex assembly using gel-based approaches.
Quantitative macromolecular binding assays including microscale thermophoresis.
The Research Specialist C is expected to develop existing methodologies to accommodate differences in experimental samples.
Qualifications: Research Specialist B: Bachelor's degree and 1 to 3 years of experience, or an equivalent combination of education and experience, are required.
Research Specialist C: Bachelor's degree and 3 to 5 years of experience, or an equivalent combination of education and experience, are required.
Reference Number: 40-27488
Salary Grade: 025
Employment Type: Exempt
Org: BB-Biochemistry and Biophysics
Job Family: I-Technical/Professional Research