Research Assistant - Luff Lab
Position Number: 00106626
Primary Function of Organization Unit: The mission of the Department of Population Health and Pathobiology is to recruit, train, inspire, and graduate Doctors of Veterinary Medicine of exemplary knowledge, skill, and character. In order to maintain the intellectual and professional climate necessary to accomplish this mission, the department's faculty will advance veterinary medical science through innovative basic and applied research, inspired mentoring of students (undergraduate, professional, residents, and graduate), excellence in clinical and diagnostic services, and innovative extension and engagement activities. North Carolina State University's College of Veterinary Medicine (CVM) is a dynamic community whose members are dedicated to preparing veterinarians and veterinarian scientists while advancing animal and human health from the cellular level through entire ecosystems. With the graduation of its first class in 1985, the CVM is one of the younger veterinary programs in the country. Despite its relative youth, the College has gained international recognition on the strength of its teaching, research, engagement, and patient care efforts and is ranked 3rd among the nation's 28 colleges of veterinary medicine in the current U.S. News & World Report survey. Located on 180 acres near downtown Raleigh, the College encompasses 20 buildings on the main Centennial Biomedical Campus. Instruction is in three departments;Clinical Sciences, Molecular Biomedical Sciences, and Population Health Pathobiology. In addition to the four-year Doctor of Veterinary Medicine degree, there are programs leading to master and doctoral degrees in several areas with numerous opportunities for specialization.
Essential Job Duties: The laboratory is focused on the mechanisms of antiviral immunity in canine and human keratinocytes and how these mechanisms are subverted by cutaneous papillomavirus oncogenes. The selected individual will knockdown genes in canine and human keratinocytes using small interfering RNAs, stimulate cells with viral analogs, and then assess expression of genes using quantitative reverse transcription PCR (RT-qPCR) and western blots. The selected individual will also be exposing keratinocytes to UVB radiation and analyzing expression of genes using RT-qPCR and western blots. This research will entail cloning of papillomavirus oncogenes into mammalian expression vectors and may involve generation of retrovirus particles for transduction of keratinocytes. The selected individual will understand the rationale for the research, and perform related experiments to test research hypotheses. Research activities include: * Management of multiple, concurrent research projects. * Method development. * Designing and carrying out experiments. * Maintenance of primary cell culture preparations. * Maintenance of primary cell culture preparations. Performance of molecular and biochemical assay, and data compilation. Procedures will include molecular and cell biological techniques on tissues collected from cell culture and animals. Research efforts will contribute toward publications and presentations.
Minimum Education/Experience: Post-Baccalaureate or Bachelor's degree in a life sciences (biology) field and experience in a research laboratory working with sterile cell cultures and molecular techniques.
Department Required Skills: * Ability to coordinate and prioritize multiple concurrent projects * Knowledge of laboratory equipment and procedure * Excellent organizational skills with strong attention to detail * Accurate record keeping and data management skills * Ability to work independently as well as contribute to a supportive team environment * Computer literacy (Microsoft Word, Excel, PowerPoint) * Excellent verbal and written communication skills * Strong analytical skills * Ability to lift up to 20 pounds.
Preferred Experience, Skills, Training/Education: * Experience in performing molecular biology experiments * Preference will be given to individuals with prior research experience, including the ability to perform basic molecular biology techniques (sterile cell culture; PCR; RT-qPCR; gel electrophoresis; basic plasmid construction and bacterial cloning; DNA, RNA, and protein extraction; western blotting; RNAi; and preparation of stock solutions)
Necessary Licenses and Certifications: N/A
AA/EOE: NC State University is an equal opportunity and affirmative action employer. All qualified applicants will receive consideration for employment without regard to race, color, national origin, religion, sex, gender identity, age, sexual orientation, genetic information, status as an individual with a disability, or status as a protected veteran.
Individuals with disabilities requiring disability-related accommodations in the application and interview process, please call 919-515-3148. Final candidates are subject to criminal & sex offender background checks. Some vacancies also require credit or motor vehicle checks. If highest degree is from an institution outside of the U.S., final candidates are required to have their degree verified at "www.wes.org":http//wes.org. Degree must be obtained prior to start date.
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