Life Science Research Professional 3
Life Science Research Professional 3
Job Number: 76591
The Ingelsson Lab in the Department of Medicine, Division of Cardiovascular Medicine at Stanford University is seeking a Life Science Research Professional 3 to perform functions and activities involved in managing major portions of complex research projects and original scientific contributions. The main focus of the Ingelsson laboratory is the link between metabolic disturbances, such as obesity and insulin resistance, and subclinical and clinical cardiovascular disease. The research is translational and interdisciplinary, combining methods from the molecular epidemiology field - such as genomic, metabolomic, transcriptomic, epigenomic and proteomic profiling in large population-based studies - with in vitro and in vivo approaches to reach new insights into the pathophysiology of cardiovascular disease and related conditions, identification of new biomarkers for improved risk prediction, and discovery of novel targets for drug development. The LSRP 3 will design, lead and perform experiments applying molecular biology, cell culture techniques, and mouse genetic models to accomplish the goals of the lab. This work will involve design and development of large-scale gene editing projects using CRISPRi, CRISPRa and CRISPR knockout libraries, as well as other molecular biology techniques, such as creation of lentiviral vectors and luciferase assays. The work will include leading cell culture experiments using CRISPR-Cas9 gene editing, siRNA and overexpression vectors, maintenance and expansion of human tissue culture cell lines and include the preparation of DNA, RNA, and protein from the samples, as well as phenotyping of cell cultures of different cell types. Mouse knockout design, maintenance and phenotyping of a mouse colony representing a variety of genetic mutations that allow manipulation of causal gene function will also be a critical component of the work to be conducted. The LSRP 3 will be expected to collect, analyze, and present research data to the research group and make original scientific contributions to the research field. Further, the work will involve troubleshooting existing protocols and development of novel protocols and procedures, as well as providing technical and scientific advice to other research personnel in lab. Duties include:
- Design research approaches for a major portion of research project(s), including background research, experimental design and execution, problem-solving, and writing up for publication.
- Develop new protocols; test and evaluate a variety of approaches.
- Perform complex (lab and/or field) experiments, and maintain detailed records of experiments and outcomes; analyze and interpret research data resulting from original procedures and methods.
- Assist with new research proposals and applications for funding and grants.
- Prepare periodic progress reports or written reports on all phases of the research project.
- Contribute substantively to the preparation of papers for publication, especially in the results section. May be a co-author on publications. Present ongoing work and findings to colleagues at academic conferences.
- Perform ongoing literature review to remain current with new procedures and related research; propose new research directions based on new advances discussed in literature.
- Oversee supply and equipment budget under minimal supervision of principal investigator or business manager.
- Work with health and safety department to ensure lab complies with regulations and all required trainings.
- Formally supervise and train new staff or students as needed, including hiring, performance management, and related duties, in addition to instruction on techniques and consultation on project work.
- Master's degree in Molecular Biology and 5 years of experience preferred.
- Proficiency in basic molecular biology techniques, including DNA and RNA purification, PCR, cell transfection and cloning.
- Proficiency in advanced molecular biology techniques, such as CRISPR gene editing including large-scale screening approaches and lentivirus techniques.
- Proficiency in cell culture techniques, such as growing and passaging cells.
- Experience with in vivo (mouse) models and mouse colony management.
- Experience with database programs for storing and tracking data/samples.
- Proficiency in scientific writing in English.
- Bachelor's degree in related scientific field and five years of related experience, or master's and two years of related experience.
- Comprehensive understanding of scientific principles.
- Expert level knowledge and skills in field of science related to research project.
- General computer skills, experience with databases and scientific applications, and ability to quickly learn and master computer programs.
- Strong analytical skills and excellent judgment.
- Ability to work under deadlines with general guidance is essential.
- Excellent organizational skills and demonstrated ability to complete detailed work accurately.
- Developing project management skills.
- Frequently stand, walk, twist, bend, stoop, squat, grasp lightly, use fine manipulation, grasp forcefully, perform desk-based computer tasks, use telephone, write by hand, lift, carry, push and pull objects weighing over 40 pounds.
- Occasionally sit, kneel, crawl, reach and work above shoulders, sort and file paperwork or parts.
- Rarely climb, scrub, sweep, mop, chop and mix or operate hand and foot controls.
- Must have correctible vision to perform duties of the job.
- Ability to bend, squat, kneel, stand, reach above shoulder level, and move on hard surfaces for up to eight hours.
- Ability to lift heavy objects weighing up to 50 pounds.
- Ability to work in a dusty, dirty, and odorous environment.
- Position may require repetitive motion.
Additional PHYSICAL REQUIREMENTS:
- May require working with low level radioactive tracers.
- May require working in close proximity to blood borne pathogens.
- May require work in an environment where animals are used for teaching and research.
- Position may at times require the employee to work with or be in areas where hazardous materials and/or infectious diseases are present.
- Employee must perform tasks that require the use of personal protective equipment, such as safety glasses and shoes, protective clothing and gloves, and possibly a respirator.
- May require extended or unusual work hours based on research requirements and business needs.
- Interpersonal Skills: Demonstrates the ability to work well with Stanford colleagues and clients and with external organizations.
- Promote Culture of Safety: Demonstrates commitment to personal responsibility and value for safety; communicates safety concerns; uses and promotes safe behaviors based on training and lessons learned.
- Subject to and expected to comply with all applicable University policies and procedures, including but not limited to the personnel policies and other policies found in the University's Administrative Guide, http://adminguide.stanford.edu.
Location: School of Medicine
To be considered for this position please visit our web site and apply on line at the following link: stanfordcareers.stanford.edu
Stanford is an equal opportunity employer and all qualified applicants will receive consideration without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, veteran status, or any other characteristic protected by law.