RESEARCH INVESTIGATOR SR

Location
Philadelphia
Posted
Aug 09, 2017
Institution Type
Four-Year Institution

Duties: The Research Investigator Sr. will be responsible for organizing and conducting research in the Pack laboratory using zebrafish and mouse models. In the zebrafish system this involves organizing and performing large scale chemical mutagenesis screen; molecular cloning of zebrafish mutants; phenotypic analyses of zebrafish mutants including histological analyses, in situ hybridization, Western blot analyses, metabolomics studies, live imaging using advanced microscopy techniques; advanced embryological analyses including microinjection and transplantation studies; advanced molecular biology methods including generation of libraries for transcriptional profiling using RNASeq, microarray analyses, both with polysome mRNA recovered from transgenic zebrafish that express ribosomal fusion proteins; bioinformatics analyses of transcriptional profiling data; generation and maintenance of transgenic lines; generation and maintenance of mutant lines using CRISPR-cas9 methods. For mouse studies this involves molecular studies for organ and cell type specific gene expression using polysome mRNA recovered from transgenic animals that express ribosomal fusion proteins; bioinformatics analyses of transcriptional profiling data; generation and maintenance of transgenic and mutant lines; tissue harvesting and recovery of specific cell types by fluorescent sorting; generation of novel mutants and transgenic animals using CRISPR-cas9 methods. This hire will assist Dr. Pack in grant writing and editing manuscripts and research grants, grant management, and the instruction and supervision of research technicians, graduate students and post-doctoral fellows performing experiments in the zebrafish and mouse model system. This hire will also be responsible for assisting Dr. Pack in coordinating ongoing multi-disciplinary projects with colleagues in other medical school departments, the School of Arts and Sciences, and outside institutions.

Qualifications: Highly advanced professional discipline. PhD or equivalent doctoral degree and at least 5 to 7 years of experience required. Experience with zebrafish and mouse models during graduate and/or postdoc training is preferred. Requirements include: expertise with zebrafish and mouse husbandry, advanced molecular biology skills, advanced microscopy skills, expertise in grant writing, and experience with lab and personnel management.

Reference Number: 40-26478

Salary Grade: RES

Employment Type: Exempt

Org: DM-Gastroenterology

Special Requirements:

Job Family: I-Technical/Professional Research