Professional Research Assistant

Aurora, CO
Jul 13, 2017
Institution Type
Four-Year Institution

University of Colorado - School of Medicine

Professional Research Assistant

The Department of Orthopedics has an opening for a full-time Professional Research Assistant/Research Associate to work at the Anschutz Medical Campus in Aurora, CO.

The successful candidate should have at least three years of experience in a molecular biology research laboratory, and should, at a minimum, possess a Bachelor's degree (Master's degree or Ph.D. preferred). This individual will be responsible for leading functional studies in zebrafish as part of a larger project investigating a genetic condition in humans.

The ideal candidate will be motivated, detail-oriented, and enthusiastic about genetic research. The individual will be supervised by a clinician scientist and instructor/lab manager. This individual will also be required to frequently interface with additional investigators who are involved with the zebrafish and functional aspects of this project. Independence and the ability to utilize university resources will be critical, as this individual will be required to perform techniques outside of the expertise of the core laboratory. Good communication skills (both verbal and written) are essential to converse with lab staff, supervisors, and collaborating investigators.

Duties include, but are not necessarily limited to:

Genetic and Molecular Biology Techniques:
  • PCR, molecular cloning and gel electrophoresis
  • Oligonucleotide design to create PCR primers and CRISPR/Cas9 sgRNAs
  • Zebrafish egg microinjection for CRISPR/Cas9 mutagenesis
  • Genomic DNA isolation from zebrafish embryos and zebrafish fin biopsies
  • Identifying and characterizing mutations using fluorescent PCR, cloning, and Sanger sequencing
  • Developing allele-specific genotyping tests and performing genotyping
  • Phenotype assessment of mutants using in situ RNA hybridization, immunohistochemistry, qPCR, microscopy, and histological staining, as needed

Zebrafish Husbandry:
  • Maintaining and caring for zebrafish stocks
  • Performing zebrafish matings to create and maintain stocks
  • Observing zebrafish for the presence of mutant phenotypes
  • Anesthetizing zebrafish and performing fin biopsies

Data Analysis and Intellectual Involvement:
  • Designing effective CRISPR/Cas9 reagents
  • Analyzing zebrafish DNA sequences for the presence of relevant mutations
  • Assisting with analysis of human next generation sequencing results, which may include background research on genes or pathway analyses
  • Researching mutations from zebrafish lines available from commercial resources (e.g. ZIRC)
  • Keeping up with relevant literature and effective laboratory techniques, especially CRISPR-Cas9 techniques

Project Leadership:
  • Communicating effectively with supervisors, collaborating scientists, and other university resources as needed
  • Supervising and training undergraduate or medical students
  • Leading the functional studies branch of the project, including assisting in the design of relevant experiments
  • Assisting in the writing of scientific literature, including manuscripts, posters, and presentations

Title selection and salary of the PRA/Research Assoc. will be dependent on education level and prior experience.

Salary and Benefits:

Salary is commensurate with skills and experience. The University of Colorado offers a full benefits package. Information on University benefits programs, including eligibility, is located at Employee Services.

The University of Colorado Denver is dedicated to ensuring a safe and secure environment for our faculty, staff, students and visitors. To assist in achieving that goal, we conduct background investigations for all prospective employees.

The University of Colorado strongly supports the principle of diversity. We encourage applications from women, ethnic minorities, persons with disabilities and all veterans. The University of Colorado is committed to diversity and equality in education and employment.

The Immigration Reform and Control Act requires that verification of employment eligibility be documented for all new employees by the end of the third day of work. Alternative formats of this ad are available upon request for persons with disabilities.


Minimum Qualifications:
  • Bachelor's degree in Biology, Biochemistry, Genetics, or a related field
  • 3+ years of experience in a Molecular Biology or Genetics laboratory

Required Competencies/Knowledge, Skills and Abilities:
  • Ability to work independently with minimal supervision
  • Excellent attention to detail, critical-thinking skills, and problem-solving abilities
  • Understanding of genetics, including genetic manipulations and CRISPR-Cas9 technologies
  • Excellent communication skills and willingness to seek guidance when necessary
  • Enthusiasm for scientific research and this project

Desired Qualifications:
  • Experience with CRISPR-Cas9 technologies
  • Experience with zebrafish husbandry and/or zebrafish genetic studies
  • Master's degree or Ph.D. in Molecular Biology, Genetics, or a related field

Special Instructions to Applicants:

Review of applications will begin July 17, 2017, and will continue until the position is filled.

Questions should be directed to

Application Materials Required:Cover Letter, Resume/CV

Application Materials Instructions:

Applications are accepted electronically at CU Careers, refer to requisition ID: 10286

When applying, applicants must include:
  1. A letter of application which specifically addresses the job requirements and outlines qualifications
  2. A current Resume/CV
  3. The names, addresses, daytime telephone numbers and e-mail addresses for three professional references, either attached to your resume/CV or uploaded as a separate document
Please be advised that the University does check references as part of the employment process.

Please do not submit any of your application material (via email) to the job posting contact.

Job Category: Faculty

Primary Location: Aurora

Department: H0001 -- Anschutz Medical Campus - 20259 - SOM-Orthopedics

Schedule: Full-time

Posting Date: Jul 12, 2017

Closing Date: Ongoing

Posting Contact Name: Erin Baschal

Posting Contact Email:

Position Number: 00050207


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