RESEARCH TECHNICIAN II

Location
Durham, NC
Posted
Jul 04, 2017
Institution Type
Four-Year Institution

diversity employer



RESEARCH TECHNICIAN II
Biostat & Bioinformatics Chairs Office

Occupational Summary

Perform a variety of complex technical duties involved in conducting high-throughput genomics experiments in support of research efforts in the Reddy Lab at Duke. The work will be in support of large-scale production style data generation projects in the lab. Work will be performed under the direction of the project lead, and will primarily report to that project lead. Details of the Reddy Lab can be viewed at http://reddylab.org.

Work Performed

Perform a variety of complex technical duties involved in completing high-throughput sequencing experiments. Tasks to be performed will include chromatin immunoprecipitation; RNA purifications; and DNA purification from cultured mammalian cells. Purified nucleic acids will then be processed into high-throughput sequencing libraries using both standard and automated molecular biology steps. Culture of mammalian cells using sterile technique; expansion of cell seed stocks; growth of cells in preparation of experiments; transfection of cells using both transient and viral approaches; treatment of cells using small molecule compounds and steroid hormones; and harvest of cells for downstream molecular analysis using but not limited to gel electrophoresis, PCR, and high-throughput sequencing. Perform routine laboratory tasks related to the above goals including preparation of reagent stocks, cell grown media, and buffers; maintaining sufficient supplies and equipment for performance of duties; cleaningand maintain laboratory equipment. Review results and quality control from completed experiments; record results in lab information management system (LIMS). Perform other tasks and special projects as assigned.

Preferred Education/Training

Work requires a bachelor's degree in biology, biochemistry, molecular biology, genetics, or other science related scientific field. Experience Previous molecular biology or biochemistry experiences a plus. Or an equivalent combination of relevant education and/or experience.

Requisition Number
401284201

Location
Durham

Duke Entity
MEDICAL CENTER

Job Code
951 RESEARCH TECHNICIAN II

Job Family Level
08

Exempt/Non-Exempt
Non-exempt

Full Time / Part Time
FULL TIME

Regular / Temporary
Regular

Shift
First/Day

Minimum Qualifications
Duke University is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, genetic information, gender, gender expression, gender identity, national origin, race, religion, sexual orientation, or veteran status. Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essential job functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.

Education

Work requires a bachelor's degree in botany, biology, zoology or other science related scientific field.

Experience

None required above education/training requirement. OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE

Auto req ID

91434BR

Duke University is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, genetic information, gender, gender expression, gender identity, national origin, race, religion, sexual orientation, or veteran status.

Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essential job functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.

PI98510870