Flow Cytometry Specialist

Location
Stanford
Posted
Jun 08, 2017
Institution Type
Four-Year Institution


Stanford University

Flow Cytometry Specialist

Job Number:
75214

Stanford University School of Medicine is seeking a Flow Cytometry Specialist (Life Science Research Professional 3) to oversee the use of multicolor flow cytometer analyzer and sorters in the Shared FACS Facility (SSFF) and Human Immune Monitoring Center (HIMC). The Stanford Shared FACS Facility located at the Beckman Center is a fee-for-service center that is heavily used to support the cell sorting and analysis needs of the School of Medicine and the rest of the University by providing training, instrument access, operator support, and new method development. This position will be shared with SSFF and HIMC.

The ideal candidate will have the ability to independently oversee the operation, maintenance, and administration of the flow cytometry resources at the SSFF satellite facility at Page Mill. In addition, they will provide local support and training for Stanford University and Parker Institute researchers at the SSFF satellite facilities. Experience with flow cytometry instrumentation (both analyzers and sorters), and ability to independently operate, maintain, and troubleshoot both analyzer and sorter instrumentation is required.

This position will require strong bench scientist skills as HIMC duties will require the candidate to perform experiments and develop protocols for cancer immunotherapy trial samples on the FACSymphony X50. Ideal candidate will have extensive experience in laboratory science - designing, implementing, and analyzing immunological experiments. Excellent communication skills and ability to juggle multiple duties is also a must. Experience in development of operator training materials and SOPs is a plus.

Duties include:
  • Become an expert user of, develop protocols for use, and independently operate a FACSymphony X-50 flow cytometer. This work includes, but is not limited to establishing sample preparation and staining protocols, properly optimize panels for spectral compensation, and population identification.
  • Independently oversee the operation of analysis and sort instrumentation for both Parker Institute projects and for the Shared FACS Facility satellites across a wide variety of biological applications. For the instrumentation this will include operation, maintenance, troubleshooting, and QC. Responsible for quality control procedures and weekly cleaning of the SSFF satellite instruments. Establish optimum running conditions.
  • Develop training protocols and train SSFF facility users in self-operation of the SSFF instruments in accordance and collaboration with other SSFF staff
  • Provide users with data analysis support for generated data, using FlowJo, SPADE, and viSNE software.
  • Understand and utilize common and unique biological applications, cell handling techniques, and reagents used by researchers and to assist them in designing and optimizing their FACS experiments;
  • Work with research staff on implementation of updates to FACS Facility instruments and computer capabilities;
  • Must be able to work a flexible schedule and to work in a BSL-2 environment to meet the needs of the facility
* - Other duties may also be assigned
DESIRED QUALIFICATIONS:

Ideal candidate will have extensive experience in bench-work science designing, implementing, and analyzing immunological experiments. In addition, experience with flow cytometry instrumentation (both analyzers and sorters), and ability to independently operate, maintain, and troubleshoot both analyzer and sorter instrumentation in an offsite facility is desired. Experience in development of operator training materials and SOPs is a plus.

EDUCATION & EXPERIENCE (REQUIRED):


Bachelor's degree in related scientific field and a minimum of five years of related experience, or master's and two years of related experience.

KNOWLEDGE, SKILLS AND ABILITIES (REQUIRED):
  • Comprehensive understanding of scientific principles.
  • Expert level knowledge and skills in field of science related to research project.
  • General computer skills, experience with databases and scientific applications, and ability to quickly learn and master computer programs.
  • Strong analytical skills and excellent judgment.
  • Ability to work under deadlines with general guidance is essential.
  • Excellent organizational skills and demonstrated ability to complete detailed work accurately.
  • Developing project management skills.
PHYSICAL REQUIREMENTS*:
  • Frequently stand, walk, twist, bend, stoop, squat, grasp lightly, use fine manipulation, grasp forcefully, perform desk-based computer tasks, use telephone, write by hand, lift, carry, push and pull objects weighing over 40 pounds.
  • Occasionally sit, kneel, crawl, reach and work above shoulders, sort and file paperwork or parts.
  • Rarely climb, scrub, sweep, mop, chop and mix or operate hand and foot controls.
  • Must have correctible vision to perform duties of the job.
  • Ability to bend, squat, kneel, stand, reach above shoulder level, and move on hard surfaces for up to eight hours.
  • Ability to lift heavy objects weighing up to 50 pounds.
  • Ability to work in a dusty, dirty, and odorous environment.
  • Position may require repetitive motion.
* - Consistent with its obligations under the law, the University will provide reasonable accommodation to any employee with a disability who requires accommodation to perform the essential functions of his or her job.

WORKING CONDITIONS:
  • May require working in close proximity to blood borne pathogens.
  • May require work in an environment where animals are used for teaching and research.
  • Position may at times require the employee to work with or be in areas where hazardous materials and/or infectious diseases are present.
  • Employee must perform tasks that require the use of personal protective equipment, such as safety glasses and shoes, protective clothing and gloves, and possibly a respirator.
  • May require extended or unusual work hours based on research requirements and business needs.
WORK STANDARDS:
  • Interpersonal Skills: Demonstrates the ability to work well with Stanford colleagues and clients and with external organizations.
  • Promote Culture of Safety: Demonstrates commitment to personal responsibility and value for safety; communicates safety concerns; uses and promotes safe behaviors based on training and lessons learned.
  • Subject to and expected to comply with all applicable University policies and procedures, including but not limited to the personnel policies and other policies found in the University's Administrative Guide, http://adminguide.stanford.edu.


Job: Research

Location: School of Medicine
Schedule: Full-time
Classification Level:

To be considered for this position please visit our web site and apply on line at the following link: stanfordcareers.stanford.edu

Stanford is an equal opportunity employer and all qualified applicants will receive consideration without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, veteran status, or any other characteristic protected by law.






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