University of Colorado - School of Medicine
This position reports directly to the lab principle investigator in the urology (Dr. Malykhina) lab, and is responsible for investigation of the mechanisms of neurogenic bladder dysfunction due to demyelination of the neural pathways innervating the lower urinary tract, as well as epigenetic changes in bladder sensory and spinal neurons. Research in the lab utilizes state-of-the-art technologies, including patch-clamp recordings in primary smooth muscle cell cultures and human cell lines, bladder innervating sensory and motor neurons, in vitro contractility studies, and pharmacogenetics in combination with in vivourodynamic assessment of bladder function. This position will supervise graduate students/fellows and report directly to Dr. Anna Malykhina, Associate Professor, Division of Urology, Department of Surgery.
- Investigate molecular mechanisms of neurogenic bladder dysfunction due to demyelination in the central nervous system using a mouse model of coronavirus-induced multiple sclerosis
- Learn a novel in vivo pharmacological approach using DREADDs for silencing bladder nociceptive neurons via AAV-driven delivery of hM4Di receptor and its Cre-dependent activation in TRPV1-cre-zGreen mice.
- Determine whether treatments that neutralize VEGF or impair the coupling between NRPs and VEGF receptors can be used to reduce bladder hyperalgesia, and normalize bladder function
- Perform experiments involving nucleic acid and protein isolation, RT-PCR and qPCR, Western blotting, use of adenoviral vectors
- Prepare data for presentations and publications
- Presentation of the data at national and international meetings
- Train graduate students and residents on lab rotations
- Attend weekly lab meetings, and individual meetings with the mentor
Additional experiments will test the role of epigenetic changes in a number of nociception-related genes modulated by transcriptional complexes such as the Methyl-CpG-binding protein 2.
- PhD (or equivalent) in biology, physiology, biochemistry, neuroscience or related discipline
- Experience in mutagenesis; tissue-specific transgenic vectors; qRT-PCR, Western Blot; in vivo Co-immunoprecipitation; Confocal microscopy, FACS, cell culture, transfections; generatiion of KO mice, genotyping.
- Excellent communication and interpersonal skills
- First author publications in peer-reviewed biomedical journals
- Experience with optogenetic approaches, and in vivo electrophysiology
Salary and Benefits:
Salary is commensurate with skills and experience. The University of Colorado offers a full benefits package. Information on University benefits programs, including eligibility, is located at Employee Services.
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Application Materials Required:Cover Letter, Resume/CV, List of References
Application Materials Instructions:
Applications are accepted electronically at CU Careers, refer to requisition ID: 09618
When applying, applicants must include:
- A letter of application which specifically addresses the job requirements and outlines qualifications
- A current Resume/CV
- The names, addresses, daytime telephone numbers and e-mail addresses for three professional references, either attached to your resume/CV or uploaded as a separate document
Please do not submit any of your application material (via email) to the job posting contact.
Job Category: Faculty
Primary Location: Aurora
Department: H0001 -- Anschutz Medical Campus - 20341 - SOM-SRG-UR DIV Clinical
Posting Date: May 22, 2017
Closing Date: Ongoing
Posting Contact Name: Kirk McCrimmon
Posting Contact Email: email@example.com
Position Number: 00695895