Post-Doctoral Fellow

Location
Aurora, CO
Posted
May 23, 2017
Institution Type
Four-Year Institution




University of Colorado - School of Medicine

Post-Doctoral Fellow

This position reports directly to the lab principle investigator in the urology (Dr. Malykhina) lab, and is responsible for investigation of the mechanisms of neurogenic bladder dysfunction due to demyelination of the neural pathways innervating the lower urinary tract, as well as epigenetic changes in bladder sensory and spinal neurons. Research in the lab utilizes state-of-the-art technologies, including patch-clamp recordings in primary smooth muscle cell cultures and human cell lines, bladder innervating sensory and motor neurons, in vitro contractility studies, and pharmacogenetics in combination with in vivourodynamic assessment of bladder function. This position will supervise graduate students/fellows and report directly to Dr. Anna Malykhina, Associate Professor, Division of Urology, Department of Surgery.

  • Investigate molecular mechanisms of neurogenic bladder dysfunction due to demyelination in the central nervous system using a mouse model of coronavirus-induced multiple sclerosis
  • Learn a novel in vivo pharmacological approach using DREADDs for silencing bladder nociceptive neurons via AAV-driven delivery of hM4Di receptor and its Cre-dependent activation in TRPV1-cre-zGreen mice.
  • Determine whether treatments that neutralize VEGF or impair the coupling between NRPs and VEGF receptors can be used to reduce bladder hyperalgesia, and normalize bladder function
  • Perform experiments involving nucleic acid and protein isolation, RT-PCR and qPCR, Western blotting, use of adenoviral vectors
  • Prepare data for presentations and publications
  • Presentation of the data at national and international meetings
  • Train graduate students and residents on lab rotations
  • Attend weekly lab meetings, and individual meetings with the mentor


Additional experiments will test the role of epigenetic changes in a number of nociception-related genes modulated by transcriptional complexes such as the Methyl-CpG-binding protein 2.

Qualifications

Minimum Requirements:

  • PhD (or equivalent) in biology, physiology, biochemistry, neuroscience or related discipline
  • Experience in mutagenesis; tissue-specific transgenic vectors; qRT-PCR, Western Blot; in vivo Co-immunoprecipitation; Confocal microscopy, FACS, cell culture, transfections; generatiion of KO mice, genotyping.
  • Excellent communication and interpersonal skills


Preferred Qualifications:

  • First author publications in peer-reviewed biomedical journals
  • Experience with optogenetic approaches, and in vivo electrophysiology


Salary and Benefits:

Salary is commensurate with skills and experience. The University of Colorado offers a full benefits package. Information on University benefits programs, including eligibility, is located at Employee Services.

The University of Colorado Denver is dedicated to ensuring a safe and secure environment for our faculty, staff, students and visitors. To assist in achieving that goal, we conduct background investigations for all prospective employees.

The University of Colorado strongly supports the principle of diversity. We encourage applications from women, ethnic minorities, persons with disabilities and all veterans. The University of Colorado is committed to diversity and equality in education and employment.

The Immigration Reform and Control Act requires that verification of employment eligibility be documented for all new employees by the end of the third day of work. Alternative formats of this ad are available upon request for persons with disabilities.



Application Materials Required:Cover Letter, Resume/CV, List of References

Application Materials Instructions:

Applications are accepted electronically at CU Careers, refer to requisition ID: 09618

When applying, applicants must include:
  1. A letter of application which specifically addresses the job requirements and outlines qualifications
  2. A current Resume/CV
  3. The names, addresses, daytime telephone numbers and e-mail addresses for three professional references, either attached to your resume/CV or uploaded as a separate document
Please be advised that the University does check references as part of the employment process.

Please do not submit any of your application material (via email) to the job posting contact.



Job Category: Faculty

Primary Location: Aurora

Department: H0001 -- Anschutz Medical Campus - 20341 - SOM-SRG-UR DIV Clinical

Schedule: Full-time

Posting Date: May 22, 2017

Closing Date: Ongoing

Posting Contact Name: Kirk McCrimmon

Posting Contact Email: kirk.mccrimmon@ucdenver.edu

Position Number: 00695895

PI97923547