Research Specialist in Chemistry - Roy J. Carver Biotechnology Center (A1700301)
Research Specialist in Chemistry
Roy J. Carver Biotechnology Center
University of Illinois- Urbana-Champaign
The Specialist will conduct protein analysis with and on behalf of Director of Proteomics
The required experience and qualifications are: M.S. in Chemistry/Biochemistry. Alternative degree fields will be considered/accepted if accompanied by equivalent experience (depending on nature and depth of experience as it relates to the position). Must have experience performing the following: protein fractionation, purification, chromatography, characterization (e.g. electrophoresis, HPLC), chemical modifications, hydrolysis (acid/alkali), enzyme digestions (e.g. traditional and microwave) and mass spectrometry analysis including MALDI ToF, ESI LC/MS, and direct infusion. Experience with protein preparation, sample cleanup (e.g. SPE, affinity column etc.), in-gel protein digestions, protein solution digestions, HPLC (e.g. preparative, analytical, capillary and nano), stable isotope tag labeling, and Orbitrap class mass spectrometers. Familiarity with using Thermo Scientific software (Xcalibur, Proteome Explorer, Matrix Sciences Mascot Distiller and Server), and Scaffold analysis and reporting software. Knowledge and understanding of protein fractionation, purification, chromatography characterization, chemical modifications, hydrolysis, enzyme digestions, and mass spectrometry, along with protein science techniques, methodologies and protocols. Effective communication, personal relations, and organizational skills. Demonstrated ability to perform effectively with a professional demeanor in a diverse, fast-paced work environment consisting of multiple and changing priorities, under minimal supervision. Ability to work both independently and as an effective team member/leader. An attention to detail, strong decision-making skills and sound judgment also required. Must have proficiency with commonly-employed software and databases.
The preferred: Ph.D. degree in Chemistry/Biochemistry. Five or more years of analytical chemistry and hands-on Proteomics experience. Additional experiences with techniques such as iTRAQ, SILAC, TMT, MaxQuant Analysis, Biacore (SPR) and proteomics LIMS.
Major duties and responsibilities:
-Provide sample cleanup (SPE and affinity column), in-gel protein digestion, protein solution digestions, hydrolysis (acid/alkali), enzyme digestions (traditional and microwave), lyophilization, HPLC (preparative, analytical, capillary, and nano), stable isotope tag labeling.
- Preparation of proteins and peptides by Fractionation, separation, measurement, and identification using electrophoresis, HPLC, FPLC and mass spectrometry.
- Perform mass spectrometric analysis using Fusion™ Orbitrap MS, MALDI ToF MS, ESI, LC/MS, Advion Triversa Nanomate robot infusion.
- Responsible for designing, performing, analysis, and training users for Surface Plasmon Resonance (Biacore) experiment.
- Responsible for providing, interpretation, and analysis of experimental results.
- Keep abreast of new equipment, materials, methodologies and techniques in protein and peptide chemistry that are relevant to the services of the facility.
- Maintain laboratory supply stock (e.g. chemicals, tube/glassware, etc.)
- Responsible for providing data to Facility Director for implementation of billing and pricing system, and serve as back-up for processing these systems.
Type of Position: Full-time Academic Professional with full University benefits package. Salary is commensurate with experience.
Application: Please complete your profile at http://jobs.illinois.edu and upload your cover letter and resume, to include at least three contacts for reference. All requested information must be submitted for your application to be considered.
For additional information, contact Dr. Peter Yau, Director of Proteomics. Phone: (217)333-3841; firstname.lastname@example.org
For full consideration, applications must be received by Friday, June 9, 2017.
The University of Illinois conducts criminal background checks on all job candidates upon acceptance of a contingent offer
The University of Illinois is an Equal Opportunity, Affirmative Action employer. Minorities, women, veterans and individuals with disabilities are encouraged to apply. For more information, visit http://go.illinois.edu/EEO. To learn more about the University's commitment to diversity, please visit http://www.inclusiveillinois.illinois.edu
Organization Name: Administration
College Name or Administrative Unit: Office of Vice Chancellor for Research
Category: Academic Professional
Open Date: 05/18/2017
Close Date: 06/08/2017