Professional Research Assistant
University of Colorado - School of Medicine
Professional Research Assistant
Division of Endocrinology, Metabolism & Diabetes is seeking a full-time Professional Research Assistant.
Nature of Work:
Primary duties will be to use Drosophila genetics to characterize organismal fat regulation. Specifically, the PRA will characterize through a combination of genetics, biochemical and behavior assays the defects of fly mutants have increased organismal body fat. This position also involves some laboratory administrational work that includes ordering and organizing lab supplies.
Technical and conceptual supervision of research program with weekly meetings; specific lists of items to be ordered and organized; specific training in new techniques.
Training new lab personnel in basic and advanced techniques, including PhD students and student workers, assigning lab duties.
Examples of Work Performed:
Drosophila melanogaster basic husbandry and genetic manipulation: tissue dissection, immunofluorescence techniques. Confocal and epifluorescense microscopy. Micromanipulation of Drosophila larvae for execution of behavior assays. Basic molecular biology and molecular cloning techniques such as: Preparation of samples by RNA, DNA extration, plasmid preps, gel electrophoresis, RT-qPCR; PCR primer design, cloning and troubleshooting and optimization of techniques. Use of sequence analysis programs. Biochemical extraction and analysis of lipids by Gas Chromatography/Mass Spectrometry. Weekly fly stock maintenance and media preparation. The candidate of this position will pursue an independent research project, and be involved in analysis of the data generated. The candidate will be required to analyze different techniques used in different labs (or described in the literature), design experiments with the PI to apply the new techniques, and adjust the scope of the project accordingly. There will be regular opportunities for presentation of results in group settings.
Salary and Benefits:
Salary is commensurate with skills and experience. The University of Colorado offers a full benefits package. Information on University benefits programs, including eligibility, is located at Employee Services.
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Knowledge, Skills and Abilities:
Prefer candidate with prior Drosophila melanogaster and/or gas chromatography mass spectrometry and/or microscopy experience. Basic molecular biology techniques, and fluorescense microscopy experience is also highly valued. In addition, applicants should be highly motivated, have excellent record-keeping and communication skills and the ability to work well in a group setting. Ability to work independently after initial training, excellent computer skills, and ability to work with minimal supervision and meet deadlines are important factors, as is experience with general lab duties including glassware cleaning, preparation of solutions and media and general laboratory maintenance.
BA/BS in biology/molecular biology/biochemistry/genetics/microbiology or a related field and significant prior research experience in molecular biology and genetics. Previous research experience with Drosophila melanogaster, basic molecular biology techniques, gas chromatography mass spectrometry and/or fluorescence microscopy. Minimum 2 year commitment, ability to work flexible hours, long-hour days, plus a few hours on weekends.
Application Materials Required:Cover Letter, Resume/CV, List of References
Application Materials Instructions:
Applications are accepted electronically at CU Careers, refer to requisition ID: 9528
When applying, applicants must include:
- A letter of application which specifically addresses the job requirements and outlines qualifications
- A current Resume/CV
- The names, addresses, daytime telephone numbers and e-mail addresses for three professional references, either attached to your resume/CV or uploaded as a separate document
Please do not submit any of your application material (via email) to the job posting contact.
Job Category: Faculty
Primary Location: Aurora
Department: H0001 -- Anschutz Medical Campus - 20184 - SOM-MED-Endocrinology
Posting Date: Jun 15, 2017
Closing Date: Ongoing
Posting Contact Name: Sabrina Ferguson
Posting Contact Email: Sabrina.email@example.com
Position Number: 00637405