RESEARCH TECHNICIAN II

Location
Durham, NC
Posted
May 16, 2017
Institution Type
Four-Year Institution

RESEARCH TECHNICIAN II
Molecular Genetics and Microbiology

Please note that this position is expected to be 100% grant funded and to last for approximately for one year. Any possible extension will be based on both funding and performance.

Occ Summary
Perform a variety of complex technical duties involved in conducting laboratory tests, experiments and determinations in support of Duke University research operations with a focus on assisting the lab in carrying out microbiome and microfluidic analyses.

Work Performed
  • Perform a variety of complex technical duties involved in conducting physical, chemical, biological and other research laboratory tests, experiments and determinations to obtain data for research purposes; compile, analyze, document and draw tentative conclusions from experimental results.
  • Confer with principal investigator to review work assignments and develop plans for research experiments; make minor modifications to existing laboratory procedures and techniques to meet the needs of the particular equipment as required.
  • Review laboratory methods manual, scientific journals, abstracts and other literature for information applicable to research experiments.
  • Determine and set up instruments, material and apparatus and operate laboratory equipment required for specific tests.
  • Obtain, prepare, mount and stain tissue, blood and/or other substances for microscopic examination.
  • Prepare culture media, chemicals, reagents and solutions.
  • Maintain sufficient inventory of material, supplies and equipment for performance of duties; clean and maintain laboratory equipment.
  • Review work of lower level laboratory personnel within designated areas and assist in training new personnel.
  • Perform other related duties incidental to the work described herein.

The above statements describe the general nature and level of work being performed by individuals assigned to this classification. This is not intended to be an exhaustive list of all responsibilities and duties required of personnel so classified.

Knowledge, Skills and Abilities
N/A

Desired skills

Experimental:
  • Anaerobic and aerobic bacterial culture
  • Bacterial DNA extraction
  • PCR
  • Agarose gel electrophoresis
  • PCR purification
  • Making bacterial freezer stock
  • Gram staining
  • Carbohydrate fermentation colorimetric assay
  • Experimental design
  • Fermenter sampling and maintenance

Computational:
  • Python
  • Matlab
  • MATLAB's Image Processing Toolbox
  • Phylogenetic software (Clustal, MAFFT, trimAl, Gblocks, PhyML, RAxML, FastTree)
  • Experience with cluster computing and job submission


Requisition Number
401257754

Location
Durham

Duke Entity
MEDICAL CENTER

Job Code
951 RESEARCH TECHNICIAN II

Job Family Level
08

Exempt/Non-Exempt
Non-exempt

Full Time / Part Time
FULL TIME

Regular / Temporary
Regular

Shift
First/Day

Minimum Qualifications
Duke University is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, genetic information, gender, gender expression, gender identity, national origin, race, religion, sexual orientation, or veteran status.Essential Physical Job Functions:Certain jobs at Duke University and Duke University Health System may include essential job functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.

Education

Work requires a bachelor's degree in botany, biology, zoology or other science related scientific field.

Experience

None required above education/training requirement. OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE

Auto req ID

89554BR

Duke University is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, genetic information, gender, gender expression, gender identity, national origin, race, religion, sexual orientation, or veteran status.

Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essential job functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.

PI97853068