Professional Research Assistant
University of Colorado - School of Medicine
Professional Research Assistant
The Division of Renal Diseases and Hypertension seeks a Professional Research Assistant to work on preclinical projects in polycystic kidney disease (PKD) and acute kidney injury (AKI). The applicant will work mainly on PKD. The applicant will perform experiments using Pkd knockout cells and genetic mouse models of PKD to study the role of the mTORC1 and 2 signaling and autophagy in mediating cyst formation and disease progression. The applicant will also learn to induce AKI by renal pedicle clamping or injection of cisplatin or lipopolysaccharide.
The PRA will run Western blots, ELISA assays, qPCR, cell culture, and flow cytometry. The PRA will manage mouse colonies of PKD mice and perform genotyping of PKD mice. The PRA will perform treatments by IP injections in PKD rats and mice. Experiments will also involve preparing slides for histologic studies and samples for immunofluorescence. PRA will measure BUN and creatinine in serum and prepare tissue samples for histology. Approximately 10% of the PRA's time will be devoted to performing routine lab work such as mouse experiments, ordering supplies, making solutions, and organizing samples and general running of the laboratory.
Salary and Benefits:
Salary is commensurate with skills and experience. The University of Colorado offers a full benefits package. Information on University benefits programs, including eligibility, is located at Employee Services.
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- Bachelor's Degree or Equivalent
- Bachelor of Science or an equivalent degree and experience with cell culture, molecular biology, biochemical techniques, and mouse handling.
- Experience working with polycystic kidney disease or similar genetic diseases.
- Experience with western blot analysis, ELISA's, qPCR, RNA isolation, flow cytometry, mouse surgery, mouse genotyping, managing mouse colonies.
Application Materials Required:Cover Letter, Resume/CV, List of References
Application Materials Instructions:
Applications are accepted electronically at CU Careers, refer to requisition ID: 9478
When applying, applicants must include:
- A letter of application which specifically addresses the job requirements and outlines qualifications
- A current Resume/CV
- The names, addresses, daytime telephone numbers and e-mail addresses for three professional references, either attached to your resume/CV or uploaded as a separate document
Please do not submit any of your application material (via email) to the job posting contact.
Job Category: Faculty
Primary Location: Aurora
Department: H0001 -- Anschutz Medical Campus - 20224 - SOM-MED-Renal General
Posting Date: May 12, 2017
Closing Date: Ongoing
Posting Contact Name: Darnell Jefferson
Posting Contact Email: Darnell.Jefferson@ucdenver.edu
Position Number: 00613707