Perform a variety of complex technical duties involved in conducting laboratory tests, experiments and determinations in support of obstetric research oriented activities at Duke University. This position will center on the characterization of labor in mouse models. In addition to ideally having mouse surgical skills, expertise in routine laboratory skills such as mouse breeding and genotyping, cell culture, cloning, microscopy, real-time PCR, western blotting, and techniques are desired.
Perform a variety of complex technical duties involved in conducting physical, chemical, biological and other research laboratory tests, experiments and determinations to obtain data for research purposes; compile, analyze, document and draw tentative conclusions from experimental results. Confer with principal investigator to review work assignments and develop plans for research experiments; make minor modifications to existing laboratory procedures and techniques to meet the needs of the particular equipment as required. Review laboratory methods manual, scientific journals, abstracts and other literature for information applicable to research experiments. Compile and document procedures and results and enter data into research database.
The project involves knowledge of mouse surgical skills. Specifically, our laboratory utilizes murine models to study labor. Mouse surgical skills are needed. We implant DSI telemeters into the uteri of pregnant mouse to monitor changes in uterine pressure during spontaneous and oxytocin-induced labor. Though the candidate likely will not have expertise in this specific procedure, we will train the candidate in the implantation of the DSI telemetry device for this purpose, but knowledge and skill-set in basic survival mouse surgery is needed. In addition, maintenance of the mouse colony is expected, thus experience with timed breedings, breeding protocols, genotyping, and mouse euthanasia is needed.
In addition, the research technician will also be required to conduct experiments from isolated murine organs to include DNA and RNA isolation, protein isolation, real-time PCR, western blotting, ELISA, and tissue mounting for microscopy. We utilize cell culture models, so experience in cell culture is desired. Lastly, we work with another laboratory in calcium imaging. If the candidate does not have experience in calcium imaging, a willingness to be trained and experience in microscopy will be needed.
The candidate will set up instruments, material and apparatus and operate standard laboratory equipment required for specific tests. They will operate various electronic devices in the collection, tabulation and calculation of data. Obtain, prepare, mount and stain tissue, blood and other substances for microscopic examinations. Prepare culture media, chemicals, reagents and solutions.
Assist in the development of new research procedures and techniques. Review work of lower level laboratory personnel and assist in the training of new personnel.
Maintain sufficient inventory of materials, supplies and equipment for performance of duties. Help maintain compliance with regulatory guidelines including Duke Biological and Chemical Safety. Complete routine laboratory cleaning and maintenance.
Perform other related duties incidental to the work described herein.
The above statements describe the general nature and level of work being performed by individuals assigned to this classification. This is not intended to be an exhaustive list of all responsibilities and duties required of personnel so classified. Employees may be directed to perform job-related tasks other than those specifically presented in this description.
691 RESEARCH SPECIALIST
Job Family Level
Full Time / Part Time
Regular / Temporary
Duke University is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, genetic information, gender, gender expression, gender identity, national origin, race, religion, sexual orientation, or veteran status. Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essential job functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.
Work requires a basic science background normally acquired through high school education.
Work requires six years of research experience OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE
Problem solving skills
Attention to detail
Experience with tissue culture and flow cytometry is preferred, but not required
Experience with sterile techniques/methods is needed
Experience in murine survival surgery is needed
Experience working in a BSL2 environment, or willingness to learn Universal Laboratory Precautions for compliance with laboratory safety and infection control procedures and practices.
Excellent interpersonal and communication skills, both written and oral
Excellent organizational skills
Data entry skills
Auto req ID
Duke University is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, genetic information, gender, gender expression, gender identity, national origin, race, religion, sexual orientation, or veteran status.
Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essential job functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.