PRA - Pietras Lab

Aurora, CO
Mar 17, 2017
Institution Type
Four-Year Institution

University of Colorado - School of Medicine

Professional Research Assistant

The Department of Medicine in the Bone Marrow Transplantation and Hematological Malignancies Program at the University of Colorado is looking for a bright and enthusiastic individual to join a research lab focused on the study of hematopoietic stem cell biology. Principal responsibilities of the position will be to be to perform key laboratory support and management tasks, manage experimental animal colonies and support the scientific program of the lab by assisting with and/or independently conducting experiments under the supervision of the PI and other laboratory staff.

Examples of Work Performed:

  • Techniques in experimental hematology and molecular biology; in vivo murine modeling; extraction and processing of organs, isolation of rare blood and blood progenitor cells from bone marrow using magnetic bead separation and flow cytometry; in vitro functional assays on blood progenitor cells; maintenance of cell cultures; primer design and DNA/RNA manipulation; quantitative PCR; molecular cloning; high throughput DNA sequencing; gene expression studies, including RNAseq; and RNAi technology. (Estimated effort: 60%)
  • Manage daily laboratory operations, including but not limited to ordering, inventory, cleaning, and preparation of laboratory reagents and husbandry/maintenance of mouse colonies. Maintain laboratory records. (Estimated effort: 35%)
  • Train students and other staff as needed, attend and present in laboratory meetings and journal clubs (Estimated effort: 5%)


  • Comply with all standard operating procedures and quality control systems
  • Comply with any laboratory duties assigned by the PI
  • Upon gaining sufficient experience, individual may train new laboratory members


Minimum Requirements:
  • BA or BS in biological sciences or related field.
  • A minimum of 1 year of laboratory experience in cellular immunology and/or molecular biology. Flow cytometry experience is greatly preferred.
  • The candidate must demonstrate a sincere desire to learn and become intellectually invested in the research program and operation of the lab with emphasis upon attention to detail; must be technically adept, highly organized, and able to maintain meticulous records of experiments, animal colonies and laboratory orders; and be able to work independently with a high degree of professionalism, sound judgment and communication skills.
  • Must be able to work effectively in team settings with others who are in peer and supervisory roles.
  • Computer proficiency is required.
  • Comply with all standard operating procedures and quality control systems, and comply with any laboratory duties assigned by the PI.

Preferred Qualifications:
  • Minimum 2-year commitment is preferred.
  • Experience and willingness to work with animal models is required, and flow cytometry sorting/analysis experience is also preferred.
  • Working knowledge of FlowJo, Microsoft Office, Prism, Adobe Photoshop and Illustrator is preferable.

Knowledge, Skills, and Abilities:

  • The ideal candidate will have an understanding of and have proficient technical skills and experience in molecular biology, immunology, genetics/genomics, and/or cancer biology.
  • Proficiency in multitasking, including managing multiple projects and laboratory duties concurrently is required.
  • Attention to detail and excellent written and communication skills are required, and candidate must have excellent organizational and record-keeping skills.
  • Computer proficiency to perform data analysis (e.g. MS Excel, Prism, FlowJo), design presentations (e.g. MS PowerPoint) and conduct word processing (e.g. MS Word) is critical.

The PRA position requires 100% effort Monday through Friday. The position requires the candidate to work flexible hours based on experimental needs, including nights on a regular basis and weekends on an occasional basis. The nature of the laboratory work generally requires time commitments that extend beyond 40 hours per week, including work conducted outside of the laboratory, such as experiment planning, data analysis and reading of scientific literature. Will be expected to actively participate in laboratory and larger group meetings and journal clubs, and to give presentations in these settings. This is an at-will exempt position and is not eligible for overtime compensation.

The above statements are intended to describe the general nature and level of the work being performed by the person assigned to this job. They are not an exhaustive list of all the potential duties and responsibilities associated with it.

Salary and Benefits:

Salary is commensurate with skills and experience. The University of Colorado offers a full benefits package. Information on University benefits programs, including eligibility, is located at Employee Services.

The University of Colorado Denver is dedicated to ensuring a safe and secure environment for our faculty, staff, students and visitors. To assist in achieving that goal, we conduct background investigations for all prospective employees.

The University of Colorado strongly supports the principle of diversity. We encourage applications from women, ethnic minorities, persons with disabilities and all veterans. The University of Colorado is committed to diversity and equality in education and employment.

The Immigration Reform and Control Act requires that verification of employment eligibility be documented for all new employees by the end of the third day of work. Alternative formats of this ad are available upon request for persons with disabilities.

Application Materials Required:Cover Letter, Resume/CV, List of References

Application Materials Instructions:

Applications are accepted electronically at CU Careers, refer to requisition ID: 8884

When applying, applicants must include:
  1. A letter of application which specifically addresses the job requirements and outlines qualifications
  2. A current Resume/CV
  3. The names, addresses, daytime telephone numbers and e-mail addresses for three professional references, either attached to your resume/CV or uploaded as a separate document
Please be advised that the University does check references as part of the employment process.

Please do not submit any of your application material (via email) to the job posting contact.

Job Category: Faculty

Primary Location: Aurora

Department: H0001 -- Anschutz Medical Campus - 20164 - SOM-MED-BoneMarrow Transplant

Schedule: Full-time

Posting Date: Mar 16, 2017

Closing Date: Ongoing

Posting Contact Name: Lynette Brandon

Posting Contact Email:

Position Number: 00732291


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