University of Colorado - School of Medicine
The major function of the Post-Doc will be performing molecular biology work including ChIP assay, siRNA work, cell culture work including primary cell lines, ZINK finger technology, RT-PCRs, Seahorse analyses, Western Blotting, and immunohistochemistry, but also to be directly involved in murine studies, including exposure of mice to myocardial-ischemia and acute lung injury induced by mechanical ventilation or LPS/Pseudomonas. Active troubleshooting, team-work and an innovative work approach are expected. In addition, the Post-Doc will also be expected to participate in general laboratory tasks, such as tissue culture work, ordering of supplies etc. Moreover, active participation in the research work, including laboratory meetings of the Eckle laboratories, reading and presenting manuscripts, work in progress presentations, and preparing research manuscripts, grants, animal protocols, or human study protocols are important aspects of the work.
The Eckle laboratory is integrated in the Cardiology Laboratories (Dr. Buttrick/Dr. Walker), the Mucosal Inflammation Program (Dr. Colgan), which represents an interdisciplinary research institute interest in understanding inflammatory diseases and the graduate training program, Department Cell Biology, Stem Cells and Development. Dr. Eckle's specific research interest is in the development of novel therapeutic approaches towards organ injury, including myocardial infarction or acute lung injury. Key aspects of Dr. Eckle's research include cardiac/lung metabolism during conditions of low oxygen availability and circadian rhythm protein function in metabolism and different diseases (heart ischemia, lung injury, delirium etc.)
The incumbent will report to Dr. Tobias Eckle, MD, PhD, Professor on a daily basis. The incumbent will receive close supervision by Dr. Eckle's staff.
After initial training in molecular biology techniques and animal surgery, the Post-Doc will also be involved in the training and supervision of more junior members of the laboratory, including medical students, graduate students or junior professional research assistants and post-docs or research fellows.
Examples of Work Performed:
- Molecular biology work including conventional reverse transcriptional polymerase chain reaction (RT-PCR) and real-time RT-PCR, protein chemistry, such as immunoprecipitation (IP), Co-IP, chromatin immunoprecipitation (ChIP) assays, generation of permanent knockdown cell lines using ZINK finger or lentivirus technology, and Western blotting; histological techniques such as tissue sections, staining and immunohistochemistry.
- Tissue culture work, including feeding and splitting of cultured endothelial, epithelial or myocardial cell lines, work with siRNA silenced cell lines and working with primary cell lines.
- Metabolic studies (Seahorse analyses, labeled tracers etc.)
- Harvesting and generation of myocardial, endothelial or fibroblast cell lines from neonatal/adult cardiac tissues.
- Harvesting and generation of lung epithelial cells from mice.
- Assisting with animal work, including myocardial, hepatic, renal ischemia, and ventilator/Pseudomonas/LPS induced lung injury or delirium in mice.
- Data gathering and documentation
- Assistance with data interpretation and analysis
- Presentation of research work and writing manuscripts or grants
Knowledge, Skills, and Abilities:
- Excellent knowledge of biological systems and molecular approaches
- Good computer skills, including experience with Microsoft Office (particularly Word , PowerPoint and Excel) and statistical software (Graph Pad Prism).
- Ability to quickly adapt to new challenges, willingness to learn, team spirit, good interaction skills.
- PhD or MD/PhD is required.
Salary and Benefits:
Salary is commensurate with skills and experience. The University of Colorado offers a full benefits package. Information on University benefits programs, including eligibility, is located at Employee Services.
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Application Materials Required:Cover Letter, Resume/CV, List of References
Application Materials Instructions:
Applications are accepted electronically at CU Careers, refer to requisition ID: 08340
When applying, applicants must include:
- A letter of application which specifically addresses the job requirements and outlines qualifications
- A current Resume/CV
- The names, addresses, daytime telephone numbers and e-mail addresses for three professional references, either attached to your resume/CV or uploaded as a separate document
Please do not submit any of your application material (via email) to the job posting contact.
Job Category: Faculty
Primary Location: Aurora
Department: H0001 -- Anschutz Medical Campus - 20138 - SOM-Anesthesiology
Posting Date: Feb 2, 2017
Closing Date: Ongoing
Posting Contact Name: Kecia Keliiholokai
Posting Contact Email: firstname.lastname@example.org
Position Number: 00731582